Expression of the neu gene-encoded protein (P185neu) in human non-small cell carcinomas of the lung.
Expression of the neu gene-encoded protein (P185neu) in human non-small cell carcinomas of the lung.
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发表时间：
1990-01
期刊：
影响因子：
11.2
通讯作者：
D. Weiner;Joanne E. Nordberg;Robert A. Robinson;P. C. Nowell;A. Gazdar;M. Greene;W. V. Williams;J. Cohen;J. Kern;J. Kern
中科院分区：
文献类型：
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作者：
D. Weiner;Joanne E. Nordberg;Robert A. Robinson;P. C. Nowell;A. Gazdar;M. Greene;W. V. Williams;J. Cohen;J. Kern;J. Kern
neu原癌基因是一个新近发现的转化基因，最初分离自乙基亚硝基脲诱导的大鼠神经母细胞瘤。我们用免疫沉淀法和免疫组化法检测了neu基因在人非小细胞肺癌中的表达。neu蛋白产物（p185 neu）存在于22个非小细胞癌细胞系中的8个。p185 neu的表达被发现在所有的组织学亚型的非小细胞癌，包括大细胞癌，鳞状细胞癌，腺癌。扩展这些数据的活检标本的人肺肿瘤表明，正常纤毛支气管上皮细胞的外周气道表达p185 neu在低水平。12例腺癌中有4例的肿瘤细胞和5例鳞癌中有3例的肿瘤细胞也表达p185 neu，表达水平高于正常纤毛支气管上皮。这些研究共同表明，p185 neu表达是人类肺肿瘤的共同特征。
The neu protooncogene is a recently described transforming gene originally isolated from ethylnitrosourea-induced rat neuroblastomas. We have examined the expression of the neu gene in human non-small cell lung carcinomas using immunoprecipitation and immunohistochemistry. The neu protein product (p185neu) was present in eight of 22 non-small cell carcinoma cell lines derived from human lung tumors. Expression of p185neu was found in all histological subtypes of non-small cell carcinomas including large cell carcinomas, squamous cell carcinomas, and adenocarcinomas. Extension of these data to biopsy specimens of human lung tumors demonstrated that normal ciliated bronchial epithelium of the peripheral airways expressed p185neu at low levels. Neoplastic cells in four of 12 adenocarcinomas and three of five squamous cell carcinomas also expressed p185neu at levels higher than the normal ciliated bronchial epithelium. Together these studies indicate that p185neu expression is a common feature of human lung tumors.