A CELL-CULTURE SYSTEM FOR SCREENING HUMAN SERUM FOR ABILITY TO PROMOTE CELLULAR CHOLESTEROL EFFLUX - RELATIONS BETWEEN SERUM COMPONENTS AND EFFLUX, ESTERIFICATION, AND TRANSFER
A CELL-CULTURE SYSTEM FOR SCREENING HUMAN SERUM FOR ABILITY TO PROMOTE CELLULAR CHOLESTEROL EFFLUX - RELATIONS BETWEEN SERUM COMPONENTS AND EFFLUX, ESTERIFICATION, AND TRANSFER
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DOI：
10.1161/01.atv.14.7.1056
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发表时间：
1994-07-01
期刊：
影响因子：
--
通讯作者：
ROTHBLAT, G
中科院分区：
文献类型：
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作者：
DELALLERAMOYA, M;ATGER, V;ROTHBLAT, G
细胞培养系统被用来测试大量的人血清样本的能力，以刺激细胞胆固醇的外排。每个标本获得的外排程度与血清胆固醇、甘油三酯、载脂蛋白（ape） B、载脂蛋白A-I、载脂蛋白A-II和脂蛋白亚组分(即高密度脂蛋白(2)[HDL（2)]、HDL(3)、脂蛋白[Lp] A-I和LpA-I:A-II）的浓度相关。此外，随后释放的胆固醇的酯化反应和合成的外源性胆固醇酯在HDL和低密度脂蛋白/极低密度脂蛋白之间的分布提供了每种血清卵磷脂：胆固醇酰基转移酶（LCAT）和胆固醇酯转移蛋白（CETP）活性的估计。分析了这些活性值与细胞外排和各种血清参数的相关性。细胞胆固醇外排与血清总高密度脂蛋白胆固醇值相关性最好。HDL(2)和HDL(3)与外排的相关性同样好，而LpA-I与外排的相关性比LpA-I: a - ii要大得多。通过偏相关分析的数据分析表明，HDL(3)和LpA-I是与外排最密切相关的HDL亚组。释放的放射性标记胆固醇的酯化与血清甘油三酯浓度呈强正相关，与血清HDL浓度呈负相关(2)。反映LCAT活性的酯化值与外排之间没有关系。标记的胆固醇酯在HDL和含载脂蛋白脂蛋白之间的转移被用作CETP活性的测量，并证明了一种模式，即所有载脂蛋白相关参数与酯化胆固醇的转移呈正相关，而所有HDL相关参数，特别是HDL(3)，与转移负相关。没有观察到外排、酯化和转移之间的关系。
A cell culture system was employed to test a large number of samples of human serum for the ability to stimulate the efflux of cell cholesterol. The extent of efflux obtained with each specimen was correlated with the serum concentrations of cholesterol, triglycerides, apoprotein (ape) B, apo A-I, apo A-II, and lipoprotein subfractions (ie, high-density lipoprotein(2) [HDL(2)], HDL(3), lipoprotein [Lp] A-I, and LpA-I:A-II). In addition, the subsequent esterification of the released cholesterol and the distribution of the synthesized exogenous cholesteryl esters between HDL and low-density lipoprotein/very-low-density lipoprotein provided estimates of the lecithin: cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) activities of each serum. The values for these activities were analyzed for correlations with cell efflux and the various serum parameters. Cell cholesterol efflux best correlated with serum total HDL cholesterol values. HDL(2) and HDL(3) correlated about equally well with efflux, whereas LpA-I demonstrated a much greater association with efflux than did LpA-I:A-II, Analysis of the data by partial correlation analysis indicated that HDL(3) and LpA-I were the HDL subfractions most closely associated with efflux. Esterification of the released radiolabeled cholesterol was strongly and positively correlated with serum triglyceride concentrations and negatively related to the serum concentrations of HDL(2). There was no relation between esterification values, which reflect LCAT activity, and efflux. The transfer of the labeled cholesteryl esters between HDL and apoB-containing lipoproteins was used as a measure of CETP activity and demonstrated a pattern in which all apoB-related parameters were positively correlated to transfer of esterified cholesterol, and all HDL associated parameters, particularly HDL(3), were negatively related to transfer. No relations were observed between efflux, esterification, and transfer.