Lipopolysaccharide-Induced Profiles of Cytokine, Chemokine, and Growth Factors Produced by Human Decidual Cells Are Altered by Lactobacillus rhamnosus GR-1 Supernatant
Lipopolysaccharide-Induced Profiles of Cytokine, Chemokine, and Growth Factors Produced by Human Decidual Cells Are Altered by Lactobacillus rhamnosus GR-1 Supernatant
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DOI：
10.1177/1933719113519171
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发表时间：
2014-07-01
影响因子：
2.9
通讯作者：
Bocking, Alan D.
中科院分区：
文献类型：
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作者：
Li, Wei;Yang, Siwen;Bocking, Alan D.
本研究的目的是评估细菌脂多糖（LPS）和鼠李糖乳杆菌GR-1上清液GR-1 SN）对人蜕膜细胞原代培养物中细胞因子、趋化因子和生长因子分泌谱的影响。脂多糖显著增加促炎细胞因子的输出（白细胞介素[IL]-1B，IL-2，IL-6，IL-12 p70，IL-15，IL-17 A，干扰素γ [IFN-γ]和肿瘤坏死因子[TNF]）;抗炎细胞因子（IL-1RN、IL-4、IL-9和IL-10）;趋化（IL-8，嗜酸性粒细胞趋化因子，IFN-诱导蛋白10 [IP-10]，单核细胞趋化蛋白1 [MCP-1]，巨噬细胞炎性蛋白-1 α [MIP-1 α]，巨噬细胞炎性蛋白-1 β [MIP-1 β]，并调节正常T细胞表达和分泌的活化[RANTES]）;和生长因子（粒细胞集落刺激因子[CSF] 3、CSF-2和血管内皮生长因子A [VEGFA]）。单独的鼠李糖乳杆菌GR-1 SN显著增加CSF-3、MIP-1 α、MIP-1 β和RANTES，但降低IL-15和IP-10的输出。GR-1 SN还显著或部分降低LPS诱导的促炎细胞因子TNF、IFN-γ、IL-1 β、IL-2、IL-6、IL-12 p70、IL-15、IL-17和IP-10;部分降低LPS诱导的抗炎细胞因子IL-IRN、IL-4和IL-10，以及LPS诱导的VEGFA输出，但不影响CSF-3、MIP-1 α、MIP-1 β、MCP-1、IL-8，IL-9。我们的研究结果表明，GR-1 SN减弱人蜕膜细胞对LPS的炎症反应，表明其在改善宫内感染中的潜在作用。
The aim of this study was to assess the effects of bacterial lipopolysaccharide (LPS) and Lactobacillus rhamnosus GR-1 supernatant GR-1SN) on secretion profiles of cytokines, chemokines, and growth factors from primary cultures of human decidual cells. Lipopolysaccharide significantly increased the output of proinflammatory cytokines (interleukin [IL]-1B, IL-2, IL-6, IL-12p70, IL-15, IL-17A, interferon gamma [IFN-gamma], and tumor necrosis factor [TNF]); anti-inflammatory cytokines (IL-1RN, IL-4, IL-9, and IL-10); chemokines (IL-8, eotaxin, IFN-inducible protein 10 [IP-10], monocyte chemoattractant protein 1 [MCP-1], macrophage inflammatory protein-1 alpha [MIP-1 alpha], macrophage inflammatory protein-1 beta [MIP-1 beta], and regulated on activation normal T cell expressed and secreted [RANTES]); and growth factors (granulocyte colony-stimulating factor [CSF] 3, CSF-2, and vascular endothelial growth factor A [VEGFA]). Lactobacillus rhamnosus GR-1SN alone significantly increased CSF-3, MIP-1 alpha MIP-1 beta, and RANTES but decreased IL-15 and IP-10 output. The GR-1SN also significantly or partially reduced LPS-induced proinflammatory cytokines TNF, IFN-gamma, IL-1 beta, IL-2 IL-6, IL-12p70, IL-15, IL-17, and IP-10; partially reduced LPS-induced anti-inflammatory cytokines IL-IRN, IL-4 and IL-10, and LPS-induced VEGFA output but did not affect CSF-3, MIP-1 alpha, MIP-1 beta, MCP-1, IL-8, and IL-9. Our results demonstrate that GR-1SN attenuates the inflammatory responses to LPS by human decidual cells, suggesting its potential role in ameliorating intrauterine infection.