Ca2+ spikes in the flagellum control chemotactic behavior of sperm
Ca2+ spikes in the flagellum control chemotactic behavior of sperm
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DOI：
10.1038/sj.emboj.7600744
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发表时间：
2005-08-03
期刊：
影响因子：
11.4
通讯作者：
Kaupp, UB
中科院分区：
文献类型：
--
作者：
Böhmer, M;Van, Q;Kaupp, UB
在精子趋化过程中发生的事件只是部分已知。作为确定潜在机制的重要一步，我们记录了海洋无脊椎动物游动精子中的Ca2+动态。刺激的海胆Arbacia punctulata的化学引诱物或细胞内cGMP引起Ca2+尖峰的鞭毛。一个Ca2+尖峰elevens一个转折的轨迹，随后一段时间的直线游泳（'转身和运行'）。Ca2+尖峰序列引起重复的环状运动。当精子在浓度梯度的引诱剂中游泳时，Ca2+尖峰和刺激功能是同步的，这表明Ca2+尖峰的精确定时控制导航。我们确定了肽asterosap作为海星Asterias amurensis的趋化因子。海星和海胆精子的Ca2+峰和游泳行为相似，这意味着趋化性的信号通路已经保守了近5亿年。
The events that occur during chemotaxis of sperm are only partly known. As an essential step toward determining the underlying mechanism, we have recorded Ca2+ dynamics in swimming sperm of marine invertebrates. Stimulation of the sea urchin Arbacia punctulata by the chemoattractant or by intracellular cGMP evokes Ca2+ spikes in the flagellum. A Ca2+ spike elicits a turn in the trajectory followed by a period of straight swimming ('turn-and-run'). The train of Ca2+ spikes gives rise to repetitive loop-like movements. When sperm swim in a concentration gradient of the attractant, the Ca2+ spikes and the stimulus function are synchronized, suggesting that precise timing of Ca2+ spikes controls navigation. We identified the peptide asterosap as a chemotactic factor of the starfish Asterias amurensis. The Ca2+ spikes and swimming behavior of sperm from starfish and sea urchin are similar, implying that the signaling pathway of chemotaxis has been conserved for almost 500 million years.