Neural progenitors from human embryonic stem cells
Neural progenitors from human embryonic stem cells
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DOI：
10.1038/nbt1201-1134
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发表时间：
2001-12-01
影响因子：
46.9
通讯作者：
Ben-Hur, T
中科院分区：
文献类型：
--
作者：
Reubinoff, BE;Itsykson, P;Ben-Hur, T
从人胚胎干细胞（ES细胞）中获得神经前体细胞在早期人类神经发生的研究和为神经移植治疗创造无限的供体细胞来源中都是有价值的。在这里，我们报告了从人ES细胞增殖神经祖细胞的富集和可扩展制剂的产生。神经前体细胞在体外可分化为星形胶质细胞、少突胶质细胞和成熟神经元。当人类神经祖细胞被移植到新生小鼠脑的脑室中时，它们大量并入宿主脑实质，表现出广泛的分布，并分化成三种神经谱系的后代。移植的细胞沿着宿主脑中已建立的脑迁移轨道迁移，并以区域特异性方式分化，表明它们可以响应局部线索并参与宿主脑发育过程。我们的观察为未来的发展奠定了基础，这可能会使人类ES细胞用于治疗神经系统疾病。
The derivation of neural progenitor cells from human embryonic stem (ES) cells is of value both in the study of early human neurogenesis and in the creation of an unlimited source of donor cells for neural transplantation therapy. Here we report the generation of enriched and expandable preparations of proliferating neural progenitors from human ES cells. The neural progenitors could differentiate in vitro into the three neural lineages-astrocytes, oligodendrocytes, and mature neurons. When human neural progenitors were transplanted into the ventricles of newborn mouse brains, they incorporated in large numbers into the host brain parenchyma, demonstrated widespread distribution, and differentiated into progeny of the three neural lineages. The transplanted cells migrated along established brain migratory tracks in the host brain and differentiated in a region-specific manner, indicating that they could respond to local cues and participate in the processes of host brain development. Our observations set the stage for future developments that may allow the use of human ES cells for the treatment of neurological disorders.