The isolation, identification and characterization of sulfated glycosaminoglycans synthesized in vitro by human eosinophils.
The isolation, identification and characterization of sulfated glycosaminoglycans synthesized in vitro by human eosinophils.
复制标题
人嗜酸性粒细胞体外合成的硫酸化糖胺聚糖的分离、鉴定和表征。
DOI：
10.1016/0304-4165(82)90359-2
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Wasserman,SI
中科院分区：
文献类型：
--
作者：
Metcalfe,DD;Litvin,J;Wasserman,SI
Human eosinophils were purified to greater than 92% using 16–30% metrizamide gradients, and these cells cultured for up to 72 h in vitro to label sulfated glycosaminoglycans. Over 90% of the sulfated glycosaminoglycan-containing material was extracted in 4 M guanidine HCl and had a hydrodynamic size similar to a glycosaminoglycan marker with an approximate average molecular weight of 60 000. Treatment of this salt-extracted35S-labeled glycosaminoglycan-containing material with 0.5 M NaOH resulted in a change in mass to approx. 20 000 daltons, suggesting that the larger molecules were proteoglycans with side chains with an approximate molecular weight of 20 000. These salt extracted presumptive35S-labeled proteoglycans were protease insensitive and behaved in a highly charged fashion on DEAE-cellulose. The composition of35S-labeled glycosaminoglycans from human eosinophils as identified using selected polysaccharidases was 70–81% chondroitin 4-sulfate, 9–12% chondroitin 6-sulfate, and 5–12% dermatan sulfate. The predominance of chondroitin 4-sulfate in human eosinophils is similar to the predominance of chondroitin 4-sulfate in human neutrophils and human platelets.
