Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins.
Photoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins.
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DOI：
10.1021/acsami.8b16736
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发表时间：
2018-11-28
影响因子：
9.5
通讯作者：
Mrksich M
中科院分区：
文献类型：
--
作者：
Zhou S;Metcalf KJ;Bugga P;Grant J;Mrksich M
This article describes a photochemical approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein bound to the surface in illuminated regions. Two different fluorescent proteins were patterned in registry with features of 400 nm in size over a 1 mm2 area. An example is given wherein an anti-carcinoembryonic antigen (anti-CEA) scFv antibody was patterned to direct the selective attachment of a human cancer cell line that express the CEA antigen. This method enables the preparation of surfaces with control over the density and activity of independently patterned proteins.
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影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
影响因子：
3.9
作者：
Alonso, Jose Maria;Reichel, Annett;del Campo, Aranzazu
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del Campo, Aranzazu
DOI：
10.1073/pnas.072685299
发表时间：
2002-04-16
影响因子：
11.1
作者：
Hodneland, CD;Lee, YS;Mrksich, M
通讯作者：
Mrksich, M
DOI：
10.1021/acs.langmuir.7b01255
发表时间：
2017-09-05
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
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作者：
El Zubir O;Xia S;Ducker RE;Wang L;Mullin N;Cartron ML;Cadby AJ;Hobbs JK;Hunter CN;Leggett GJ
通讯作者：
Leggett GJ
DOI：
10.1083/jcb.200810041
发表时间：
2009-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doyle AD;Wang FW;Matsumoto K;Yamada KM
通讯作者：
Yamada KM