Protective effect of polysaccharide from Sophora japonica L. flower buds against UVB radiation in a human keratinocyte cell line (HaCaT cells)
Protective effect of polysaccharide from Sophora japonica L. flower buds against UVB radiation in a human keratinocyte cell line (HaCaT cells)
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DOI：
10.1016/j.jphotobiol.2018.12.001
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发表时间：
2019-02-01
影响因子：
5.4
通讯作者：
Dou, Yanli
中科院分区：
文献类型：
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作者：
Li, Liyan;Huang, Tao;Dou, Yanli
天然植物提取物在皮肤抗紫外线（UV）辐射领域最近引起了相当大的关注。国槐花蕾是一种药用植物，含有黄酮类、生物碱类、三萜类、多糖类等多种成分，除止血外，还具有多种药理作用。本研究的目的是探讨槐米多糖提取物是否具有生物活性。花蕾（PS）能够使用人角质形成细胞系（HaCaT细胞）减弱UVB诱导的损伤。HaCaT细胞在无血清培养基中用PS预处理2 h，然后用不同剂量的UVB射线照射。结果表明，PS对UVB诱导的细胞毒性有一定的抑制作用，MTT法和形态学特征分析证实了PS的抑制作用。UVB照射（30-120 mJ/cm（2））24 h后，随着照射剂量的增加，HaCaT细胞活力显著降低; PS（0.25-2.0 mg/mL）预处理可显著降低UVB对HaCaT细胞的毒性，并呈剂量依赖性地提高HaCaT细胞的活力。PS通过MAPK途径抑制UVB照射后HaCaT细胞ROS的产生，下调磷酸化JNK和磷酸化p38 MAPK蛋白的表达。低剂量UVB照射后HaCaT细胞凋亡率明显降低，Caspase 3活性降低，提示其对UVB照射后HaCaT细胞凋亡有保护作用。结论：PS预处理能有效保护HaCaT细胞免受UVB辐射损伤，其机制可能与MAPK信号通路参与细胞凋亡有关。然而，需要进一步的研究，特别是使用人体系统来确定PS在体内的功效。
Natured botanical extract has attracted considerable attention recently in the field of skin anti-ultraviolet (UV) radiation. As a medicinal herb, Sophora japonica flower buds contained several components such as flavonoids, isoflavonoids, triterpenes, alkaloids and polysaccharides, which have multiple pharmacological properties except hemostatic agents which have been used in China and Korea for centuries. The purpose of our study was to investigate whether polysaccharide extracted from Sophora japonica L. flower buds (PS) was able to attenuate UVB-induced damage using a human keratinocyte cell line (HaCaT cells). HaCaT cells were pretreated with PS in a serum-free medium for 2 h and then irradiated with different doses of UVB rays. The results showed that the PS attenuated UVB-induced cytotoxicity which was verified by MTT method and morphology feature assay. UVB exposure (30-120 mJ/cm(2)) reduced HaCaT cells viability significantly following with the increased irradiation dose 24 h later, while pretreatment with PS (0.25-2.0 mg/mL) attenuated UVB-induced cytotoxicity significantly and increased cell viability in a dose-dependent manner except 30 mJ/cm(2) group. The PS reduced the ROS generation, down-regulated the expression of phosphor-JNK and phosphor-p38 MAPK proteins significantly through MAPK pathway in UVB-irradiated HaCaT cells. It also decreased the apoptosis rate at low dose of UVB ray and protected the cells from apoptosis which had been identified by the down-regulated level of active-caspase3 in UVB-irradiated HaCaT cells. In conclusion, PS pretreatment protected HaCaT keratinocytes from UVB irradiation-induced skin injuries effectively, and the underlying mechanism may involve MAPK signaling pathway which contribute to apoptotic cell death. However, further studies especially whose using human systems are needed to determine efficacy of PS in vivo.