Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells
Defining a developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/progenitor cells
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DOI：
10.1634/stemcells.2005-0332
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发表时间：
2006-04-01
期刊：
影响因子：
5.2
通讯作者：
Ko, Minoru S. H.
中科院分区：
文献类型：
--
作者：
Aiba, Kazuhiro;Sharov, Alexei A.;Ko, Minoru S. H.
为了了解体外神经分化过程中基因表达变化的整体特征，我们对胚胎干细胞（ESCs）、胚胎癌细胞和成体神经干细胞/祖细胞（NS）进行了微阵列分析。在单层培养中，ESCs在分化过程中的表达谱显示出三个不同的阶段：未分化的ESCs，原始外胚层样细胞和神经祖细胞。主成分（PC）分析显示，这些细胞在PC1上排列了6天。这个PC1代表了大约4000个基因，其表达随着神经承诺/分化而增加。此外，与胚胎干源性神经祖细胞相比，来自成人脑的神经干细胞及其分化细胞沿着PC轴定位，距离未分化的ESCs更远。我们认为这种PC1定义了神经命运的路径，为承诺/分化的程度提供了一个尺度。
To understand global features of gene expression changes during in vitro neural differentiation, we carried out the microarray analysis of embryonic stem cells (ESCs), embryonal carcinoma cells, and adult neural stem/progenitor (NS) cells. Expression profiling of ESCs during differentiation in monolayer culture revealed three distinct phases: undifferentiated ESCs, primitive ectoderm-like cells, and neural progenitor cells. Principal component (PC) analysis revealed that these cells were aligned on PC1 over the course of 6 days. This PC1 represents approximately 4,000 genes, the expression of which increased with neural commitment/differentiation. Furthermore, NS cells derived from adult brain and their differentiated cells were positioned along this PC axis further away from undifferentiated ESCs than embryonic stem-derived neural progenitors. We suggest that this PC1 defines a path to neural fate, providing a scale for the degree of commitment/differentiation.