Comparative Study of the Pharmacokinetic Parameters for Salidroside in Normal and Estrogen-Deficient Female Rats after Oral Administration of an Aqueous Extract of Fructus Ligustri Lucidi using a Validated Ultra-Performance Liquid Chromatography Mass Spectrometry/Mass Spectrometry Method
Comparative Study of the Pharmacokinetic Parameters for Salidroside in Normal and Estrogen-Deficient Female Rats after Oral Administration of an Aqueous Extract of Fructus Ligustri Lucidi using a Validated Ultra-Performance Liquid Chromatography Mass Spectrometry/Mass Spectrometry Method
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使用经验证的超高效液相色谱质谱法对正常和雌激素缺乏雌性大鼠口服女贞子水提取物后红景天苷的药代动力学参数进行比较研究
DOI：
10.4103/pm.pm_278_19
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发表时间：
2020-07-01
影响因子：
0.7
通讯作者：
Zhang, Dongwei
中科院分区：
文献类型：
--
作者：
Chen, Beibei;Tang, Jinfa;Zhang, Dongwei
背景：红景天苷是女贞子（FLL）中的主要活性成分之一，具有良好的抗骨质疏松作用。然而，雌激素缺乏大鼠FLL中红景天苷的血浆药代动力学特征尚不清楚。目的：建立一种灵敏、快速、准确的超高效液相色谱-质谱（UPLC-MS）方法，用于测定红景天苷在正常大鼠和去卵巢大鼠体内的药动学特征。材料与方法：以7 g/kg的剂量给药OVX和正常大鼠。血浆样品用甲醇沉淀，上清用Waters BEH C-18色谱柱层析，乙酸铵和乙腈梯度洗脱。定量分析了电喷雾电离、正多反应监测模式。结果：检测下限为50 ng/mL，动态线性范围为50 ~ 30000 ng/mL， R-2 > 0.99。日内、日间精密度均低于14.67%，准确度在99.29% ~ 103.37%之间。红景天苷的回收率为88.90% ~ 101.78%，基质效应为85.53% ~ 100.45%。OVX大鼠红景天苷的t1 /2、MRT0-8和表观分布体积增加。结论：建立了一种灵敏、准确、快速的测定SD大鼠红景天苷血浆特征的方法。提示卵巢切除可影响SD大鼠红景天苷代谢。
Background: Salidroside, one of the main active ingredients in Fructus Ligustri Lucidi (FLL), is well demonstrated to exert anti-osteoporotic effect. However, the plasma pharmacokinetic profile of salidroside in FLL in estrogen-deficient rats remains unknown. Objective: The objective was to develop a sensitive, rapid, and accurate ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for the determination of the pharmacokinetics profile of salidroside after oral administration of FLL aqueous extract in normal and ovariectomized (OVX) rats. Materials and Methods: OVX and normal rats were orally administrated with FLL at a bolus of 7 g/kg. Plasma samples were precipitated by methanol, and the supernatant was chromatographed by a Waters BEH C-18 column with a gradient elution of ammonium acetate and acetonitrile. Quantification was carried out on the electrospray ionization, positive multiple reaction monitoring modes. Results: The lower limit of detection was 50 ng/mL, and the dynamic linear range was 50-30,000 ng/mL with a value of R-2 > 0.99. The intra- and inter-day precisions were lower than 14.67%, and accuracy was in the range of 99.29%-103.37%. The recovery of salidroside ranged from 88.90% to 101.78%, with the matrix effect ranging from 85.53% to 100.45%. The t 1/2, MRT0-8, and apparent volume of distribution for salidroside increased in OVX rats. Conclusion: A sensitive, accurate, and rapid method was successfully established and validated for the determination of plasma characteristics of salidroside in Sprague-Dawley (SD) rats. The results suggest that ovariectomy could interfere with salidroside metabolism in SD rats.