Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance
Modification of cellulose thin films with lysine moieties: a promising approach to achieve antifouling performance
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DOI：
10.1007/s10570-017-1538-9
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发表时间：
2018-01-01
期刊：
影响因子：
5.7
通讯作者：
Kleinschek, Karin Stana
中科院分区：
文献类型：
--
作者：
Elschner, Thomas;Bracic, Matej;Kleinschek, Karin Stana
采用旋涂法制备了三甲基硅基纤维素薄膜，并将其再生为纤维素。表面用N，N‘-甲酰基二咪唑活化，在水溶液中与赖氨酸进行氨解反应，得到赖氨酸氨基甲酸酯纤维素膜。用ATR-IR光谱、零电势测量、接触角测量和原子力显微镜对表面进行了分析表征。官能团的量由pH电位滴定和金刚三酮试验测定，范围为25pmolcm(-2)。用耗散监测石英晶体微天平(QCM-D)研究了不同pH值条件下牛血清白蛋白(BSA)和纤维蛋白原(Fg)在纤维素膜和纤维素赖氨酸氨基甲酸酯表面的吸附。赖氨酸修饰显著降低了牛血清白蛋白的吸附。在生理pH值(7.4)下，纤维蛋白原的吸附比纯纤维表面低6倍(1.0mGM(-2))。因此，含赖氨酸基团的纤维素薄膜有望成为血液接触设备领域中血液相容防污表面的候选材料。
Thin films of trimethylsilyl cellulose are obtained by spin coating and regenerated to cellulose. The surface is activated with N,N'-carbonyldiimidazole and aminolysis with lysine is carried out in aqueous solution to yield a cellulose lysine carbamate film. The surface is analytically characterized by ATR-IR spectroscopy, zetapotential measurements, contact angle measurements, and atomic force microscopy. The amount of functional groups is determined by pH potentiometric titration as well as the ninhydrin test and is in the range of 25 pmolcm(-2). Adsorption of bovine serum albumine (BSA) and fibrinogen on the cellulose film and the cellulose lysine carbamate surface is studied at different pH values by quartz crystal microbalance with dissipation monitoring (QCM-D). The adsorption of BSA is significantly reduced by modification with lysine. At physiological pH value (7.4) the adsorption of fibrinogen is even six times lower (1.0 mgm(-2)) compared to the pure cellulose surface. Thus, cellulose thin films with lysine moieties are promising candidates for hemo-compatible antifouling surfaces in the field of blood contacting devices.