ISOLATION AND CHARACTERIZATION OF A NOVEL TROPHOBLAST-SPECIFIC CDNA IN THE MOUSE
ISOLATION AND CHARACTERIZATION OF A NOVEL TROPHOBLAST-SPECIFIC CDNA IN THE MOUSE
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DOI：
10.1101/gad.2.12a.1639
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发表时间：
1988-12-01
影响因子：
10.5
通讯作者：
ROSSANT, J
中科院分区：
文献类型：
--
作者：
LESCISIN, KR;VARMUZA, S;ROSSANT, J
通过对小鼠13.5天胎盘cDNA文库的差异筛选，分离到一个新的滋养层特异性cDNA。该cDNA全长755 bp，编码124个氨基酸的多肽，含有一个18个氨基酸的疏水前导序列。与任何已知的基因或蛋白质没有显著的同源性。北方印迹分析表明，该基因在胎盘中大量表达，而在任何其他组织中均未检测到。原位杂交显示，该基因首先在发育7.5天的胎盘外锥细胞的一个亚组中表达，其后期表达仅限于成熟胎盘的海绵滋养层细胞。该基因的作用目前尚不清楚，但信号肽的存在表明它可能是一种分泌蛋白。
A novel trophoblast-specific cDNA has been isolated by differential screening of a 13.5-day mouse placental cDNA library. The 755-bp cDNA, which is almost full length, encodes a polypeptide of 124 amino acids with an 18-amino-acid hydrophobic leader sequence. No significant homology with any known gene or protein has been detected. Northern blot analysis has shown that the gene is expressed abundantly in the placenta and is not detected in any other tissues. In situ hybridization revealed that the gene is expressed first in a subgroup of ectoplacental cone cells at 7.5 days of development and its later expression is confined to the spongiotrophoblast cells of the mature placenta. The role of the gene is not currently known, but the presence of a signal peptide suggests that it may be a secreted protein.