Inonotus obliquus polysaccharide ameliorates dextran sulphate sodium induced colitis involving modulation of Th1/Th2 and Th17/Treg balance
Inonotus obliquus polysaccharide ameliorates dextran sulphate sodium induced colitis involving modulation of Th1/Th2 and Th17/Treg balance
复制标题
桦褐孔菌多糖改善葡聚糖硫酸钠诱导的结肠炎，涉及调节 Th1/Th2 和 Th17/Treg 平衡
DOI：
10.1080/21691401.2019.1577877
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Jin, Dan
中科院分区：
文献类型：
--
作者：
Chen, Yi-Fang;Zheng, Jin-Juan;Jin, Dan
炎症性肠病（Inflammatory Bowel Disease，IBD）是一种肠道慢性炎症性疾病，与CD 4（+）T细胞亚群失衡有关。然而，目前慢性结肠炎的治疗方法并不理想，并且有潜在的副作用。因此，从天然植物中提取更有效、更安全的生物活性物质受到广泛关注。本研究发现，桦褐孔菌的主要活性成分桦褐孔菌多糖（IOP）对葡聚糖硫酸钠诱导的小鼠慢性肠道炎症有一定的缓解作用。经口给予IOP（100、200、300 mg/kg）可显著降低结肠炎小鼠的疾病活动指数，减轻结肠组织的病理改变，其中紧密连接蛋白Occludin和ZO-1的丢失减少。逆转录-聚合酶链反应（RT-PCR）和流式细胞术检测表明，它还能调节结肠组织、肠系膜淋巴结和脾脏中失衡的Th 1/Th 2和Th 17/Treg。免疫组化和western blot进一步显示IOP对慢性结肠炎小鼠结肠组织p-STAT 1、p-STAT 6、p-STAT 3表达的调节作用，促进了Th 1/Th 2、Th 17/Treg的平衡。总之，这些结果表明IOP是IBD的潜在有效治疗剂。
Inflammatory bowel disease (IBD) is an intestinal chronic inflammatory disease, and is related to imbalance of CD4(+)T subsets. However, the current treatments of chronic colitis are not ideal and have potential side effects. Therefore, more effective and safer biologically active substances which are extracted from natural plants have been widely concerned. In this study, it was found that Inonotus obliquus polysaccharides (IOP), the main bioactive constituent of Inonotus obliquus, can alleviate dextran sodium sulfate-induced chronic murine intestinal inflammation. Oral administration of IOP (100, 200, 300 mg/kg) can significantly reduce the disease active index and alleviate the pathological changes in colitis mice, where the tight junction proteins Occludin and ZO-1 losses in colon tissues were reduced. It can also regulate imbalanced Th1/Th2 and Th17/Treg in colon tissues, mesenteric lymph nodes and spleen using Reverse Transcription-Polymerase Chain Reaction detection and flow cytometry. Immunohistochemistry and western blot assays further revealed the modulatory effect of IOP on the p-STAT1, p-STAT6, p-STAT3 expression, which promoted the balance of Th1/Th2, Th17/Treg in the colon of chronic colitis mice. In short, these results indicated that IOP was potentially effective therapeutic agent for IBD.