Progress in transduction of cerebellar Purkinje cells in vivo using viral vectors
Progress in transduction of cerebellar Purkinje cells in vivo using viral vectors
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DOI：
10.1007/s12311-008-0012-5
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发表时间：
2008-09-01
期刊：
影响因子：
3.5
通讯作者：
Hirai, Hirokazu
中科院分区：
文献类型：
--
作者：
Hirai, Hirokazu
外源基因在体内小脑浦肯野细胞中的表达是研究小脑病理生理学的一种有效方法。虽然使用发育工程已经产生了许多基因修饰的小鼠，但这种方法是耗时的，并且需要大量的努力来交叉不同品系的小鼠，基因分型和动物的维护。如果感兴趣的基因可以转移到发育和成熟动物的浦肯野细胞中并在其中有效表达，则可以节省大量的时间，精力和金钱。病毒载体的最新进展显着有助于选择性和有效的基因转移到浦肯野细胞在体内。有两种方法用于在浦肯野细胞中选择性基因表达：一种是利用病毒对浦肯野细胞的嗜性，其包括腺相关病毒和水泡性口炎病毒糖蛋白（VSV-G）假型慢病毒的嗜性。可以与第一种方法组合使用的另一种方法是利用浦肯野细胞特异性启动子。本文就病毒载体介导的浦肯野细胞体内转导的研究进展作一综述。
Expression of a foreign gene in cerebellar Purkinje cells in vivo is a powerful method for exploring the pathophysiology of the cerebellum. Although using developmental engineering many gene-modified mice have been generated, this approach is time-consuming and requires a lot of effort for crossing different lines of mice, genotyping and maintenance of animals. If a gene of interest can be transferred to and efficiently expressed in Purkinje cells of developing and mature animals, it saves much time, effort and money. Recent advances in viral vectors have markedly contributed to selective and efficient gene transfer to Purkinje cells in vivo. There are two approaches for selective gene expression in Purkinje cells: one is to take advantage of the viral tropism for Purkinje cells, which includes the tropism of adeno-associated virus and the vesicular stomatitis virus glycoprotein (VSV-G)-pseudotyped lentivirus. Another method, which might be used in combination with the first one, is utilization of a Purkinje-cell-specific promoter. Focusing mainly on these points, recent progress in viral-vector-mediated transduction of Purkinje cells in vivo is reviewed.