A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees.
A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees.
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DOI：
10.3390/insects8010028
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发表时间：
2017-03-07
期刊：
影响因子：
3
通讯作者：
Martin SJ
中科院分区：
文献类型：
--
作者：
Brettell LE;Mordecai GJ;Schroeder DC;Jones IM;da Silva JR;Vicente-Rubiano M;Martin SJ
变形翅病毒(DWV)与瓦罗亚破坏因子(Varroa Destructor)有关，目前被认为是西方蜜蜂群体崩溃的罪魁祸首。长期以来，在受感染的蜂群中出现畸形个体一直与蜂群的丧失有关。然而，目前还不清楚为什么只有一小部分DWV阳性的蜜蜂出现了畸形的翅膀。这项研究涉及两项比较变形和非变形蜜蜂的小型研究。在巴西，被Varroa寄生为蛹的无症状蜜蜂(没有翅膀畸形)的DWV负荷比未被寄生的蜜蜂高。然而，我们没有发现由于DWV滴度或寄生而在翅膀形态上更大的两侧不对称。正如预期的那样，使用RT-qPCR，畸形蜜蜂被发现含有最高的病毒载量。在另一项研究中，下一代测序(NGS)被应用于比较来自两个不同夏威夷岛屿上的三个蜂群的成对的有症状和无症状的蜜蜂的整个DWV基因组。这表明来自畸形或无症状蜜蜂的DWV基因组之间没有一致的差异，最大的差异出现在位置之间，而不是表型。除了一个样本外，所有样本都以Dwv A型为主。这项小规模研究表明，没有与翅膀畸形有关的独特遗传变异；但许多Dwv变异有可能导致畸形。
Deformed wing virus (DWV) in association with Varroa destructor is currently attributed to being responsible for colony collapse in the western honey bee (Apis mellifera). The appearance of deformed individuals within an infested colony has long been associated with colony losses. However, it is unknown why only a fraction of DWV positive bees develop deformed wings. This study concerns two small studies comparing deformed and non-deformed bees. In Brazil, asymptomatic bees (no wing deformity) that had been parasitised by Varroa as pupae had higher DWV loads than non-parasitised bees. However, we found no greater bilateral asymmetry in wing morphology due to DWV titres or parasitisation. As expected, using RT-qPCR, deformed bees were found to contain the highest viral loads. In a separate study, next generation sequencing (NGS) was applied to compare the entire DWV genomes from paired symptomatic and asymptomatic bees from three colonies on two different Hawaiian islands. This revealed no consistent differences between DWV genomes from deformed or asymptomatic bees, with the greatest variation seen between locations, not phenotypes. All samples, except one, were dominated by DWV type A. This small-scale study suggests that there is no unique genetic variant associated with wing deformity; but that many DWV variants have the potential to cause deformity.