Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles
Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles
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DOI：
10.1038/ncomms5462
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发表时间：
2014-07-01
影响因子：
16.6
通讯作者：
Yang, Liang
中科院分区：
文献类型：
--
作者：
Chua, Song Lin;Liu, Yang;Yang, Liang
细菌在浮游和生物膜生长模式期间呈现不同的生活方式。细胞内信使c-di-GMP水平的增加决定了从增殖到生物膜生长的转变，而减少则导致生物膜分散。通常认为，从生物膜分散的细胞立即进入增殖生长期。在这里，我们使用单核苷酸分辨率转录组学分析表明，分散的细胞从铜绿假单胞菌生物膜的生理是非常不同的，从那些嗜酸性和生物膜细胞。在分散的细胞中，小调节RNA RsmY和RsmZ的表达下调，而分泌基因被诱导。分散的细胞对巨噬细胞和秀丽隐杆线虫的毒性比嗜热细胞强。此外，它们对铁胁迫高度敏感，并且生物膜分散剂、铁螯合剂和妥布霉素的组合有效地降低了分散细胞的存活。
Bacteria assume distinct lifestyles during the planktonic and biofilm modes of growth. Increased levels of the intracellular messenger c-di-GMP determine the transition from planktonic to biofilm growth, while a reduction causes biofilm dispersal. It is generally assumed that cells dispersed from biofilms immediately go into the planktonic growth phase. Here we use single-nucleotide resolution transcriptomic analysis to show that the physiology of dispersed cells from Pseudomonas aeruginosa biofilms is highly different from those of planktonic and biofilm cells. In dispersed cells, the expression of the small regulatory RNAs RsmY and RsmZ is downregulated, whereas secretion genes are induced. Dispersed cells are highly virulent against macrophages and Caenorhabditis elegans compared with planktonic cells. In addition, they are highly sensitive towards iron stress, and the combination of a biofilm-dispersing agent, an iron chelator and tobramycin efficiently reduces the survival of the dispersed cells.