A DNA nanomachine powered by light irradiation
A DNA nanomachine powered by light irradiation
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DOI：
10.1002/cbic.200700649
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发表时间：
2008-03-25
期刊：
影响因子：
3.2
通讯作者：
Asanuma, Hiroyuki
中科院分区：
文献类型：
--
作者：
Liang, Xingguo;Nishioka, Hidenori;Asanuma, Hiroyuki
在过去的十年中，DNA已被广泛用于纳米材料的ACHTUNGTRENNUNG开发，因为它经历了高度ACHTUNGTRENNUNG序列特异性杂交，并形成具有适当柔性的高度规则的双螺旋结构。[1-4]DNA可能是未来在纳米技术和材料科学中应用最有前途的生物分子之一。[5]近年来，以DNA为构筑材料的二维和三维纳米结构被广泛报道。[3，6，7]更令人兴奋的是，已经构建了几种类型的DNA纳米机器，以DNA寡核苷酸[8]或其他分子如嵌入剂[9]和金属离子[10]为燃料。[4，5]在这10年的发展过程中，基于DNA的装置（如镊子、行走器和齿轮）的设计取得了实质性进展，这些装置可以执行切割、定向运动或滚动等机械功能。[11-13]这一领域的前景是非常有希望的，DNA纳米机器作为传感器，转运体和药物递送系统的一些有价值的应用也已报道。[5]对于迄今为止构建的大多数DNA纳米机器，寡核苷酸通常用作燃料。在许多这些系统中，机械运动通常通过将一个DNA燃料分子与靶序列杂交，然后用与第一个完全或部分互补的另一个DNA序列将其去除来进行。[5]Yurke埃塔尔展示了第一台DNA机器，它的功能就像“镊子”，由两条具有定制互补性的DNA链驱动。[8a]由于操作这些DNA纳米机器的能量是通过链交换策略产生的，因此在每个工作循环中都会产生DNA双链体作为废物。因此，随着“废物”的积累，运行效率逐渐降低。因此，需要一个新的策略来克服这个问题，为进一步发展的DNA nanotechnology. In过去的十年中，我们已经开发了一系列的光响应的DNA通过共价连接偶氮苯部分到DNA链。[14-19]这些光响应DNA与单链DNA（以形成双链体）、RNA（以形成DNA-RNA杂交体）或双链DNA（以形成三链体）的杂交。
Over the past decade, DNA has been widely used for the ACHTUNGTRENNUNGdevelopment of nanomaterials because it undergoes highly ACHTUNGTRENNUNGsequence-specific hybridization and forms a highly regular double-helical structure with suitable flexibility.[1–4] DNA is probably one of the most promising biomolecules for future applications in nanotechnology and materials science.[5] Many 2D and 3D nanostructures with determined shapes and geometries have been reported recently in which DNA is used as the building blocks and mortar.[3, 6, 7] More excitingly, several types of DNA nanomachines, fuelled with DNA oligonucleotides [8] or other molecules such as intercalators [9] and metal ions,[10] have been constructed.[4, 5] During these 10 years of development, substantial progress has been made in the design of DNA-based devices such as tweezers, walkers, and gears, which can perform mechanical functions such as scission, directional motion, or rolling.[11–13] The prospects of this field are extraordinarily promising, and several valuable applications of DNA nanomachines as sensors, transporters, and drug-delivery systems have also been reported.[5] For most of the DNA nanomachines constructed so far, oligonucleotides have been generally used as the fuel. In many of these systems, the mechanical motion was usually carried out by hybridization of one DNA fuel molecule to target sequences followed by its removal with another DNA sequence that is completely or partially complementary to the first.[5] Yurke etal. demonstrated the first DNA machine that functioned as “tweezers” fuelled by two strands of DNA with tailored complementarity.[8a] As the energy for operating these DNA nanomachines is produced by a strand-exchange strategy, a DNA duplex is produced as a waste product in every working cycle. Thus, the operating efficiency decreases gradually with the accumulation of “wastes”. A new strategy is therefore required to overcome this problem for the further development of DNA nanotechnology.Over the past decade, we have developed a series of photoresponsive DNAs by covalently tethering azobenzene moieties onto the DNA strand.[14–19] Hybridization of these photoresponsive DNAs to single-stranded DNA (to form duplexes), RNA (to form DNA–RNA hybrids), or double-stranded DNA (to form tri-