Augmentation of stimulated eosinophil degranulation by VLA-4 (CD49d)-mediated adhesion to fibronectin.
Augmentation of stimulated eosinophil degranulation by VLA-4 (CD49d)-mediated adhesion to fibronectin.
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VLA-4 (CD49d) 介导的纤连蛋白粘附增强了刺激的嗜酸性粒细胞脱颗粒。
DOI：
10.1165/ajrcmb.11.2.8049081
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发表时间：
1994
影响因子：
6.4
通讯作者：
Leff,AR
中科院分区：
文献类型：
--
作者：
Neeley,SP;Hamann,KJ;Dowling,TL;McAllister,KT;White,SR;Leff,AR
We examined the effect of VLA-4-mediated adhesion to purified fibronectin (FN) on the stimulated release of the granular protein, eosinophil peroxidase (EPO), in human peripheral blood eosinophils. In initial studies, optimal time-course and concentration-effect relationships were determined; eosinophil adhesion to FN-coated styrene plates was maximal in wells coated with 10 micrograms/ml FN after incubation in the wells for 60 min (17,097 +/- 3,670 adherent eosinophils/well versus 6,789 +/- 925 adherent eosinophils/well in control wells; P < 0.005). Treatment of eosinophils with 10(-8) to 10(-6) M formylmethionylleucyl-phenylalanine (FMLP) + 5 micrograms/ml cytochalasin B (CYTB) caused a concentration-dependent increase in EPO release, which was augmented by preincubation of eosinophils for 120 min in FN-coated (10 micrograms/ml) styrene wells versus eosinophils preincubated in control wells. At 10(-6) M FMLP+CYTB, initial adhesion to FN for 120 min caused an increase in the secretion of EPO from 367 +/- 26 to 485 +/- 25 ng/10(6) eosinophils (P = 0.0001). Treatment of eosinophils during incubation in FN-coated wells with the anti-VLA-4 monoclonal antibody HP2/1 attenuated stimulated EPO secretion caused by 10(-6) M FMLP+CYTB from 497 +/- 40 to 285 +/- 26 ng/10(6) eosinophils (P < 0.02). Similarly, treatment with HP2/1 caused a decrease in eosinophil adhesion to FN-coated styrene from 12,693 +/- 1,866 to 6,206 +/- 852 adherent cells/FN-coated well (P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
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细胞因子对嗜酸性粒细胞脱颗粒的调节作用。
DOI：
--
发表时间：
1990
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fujisawa,T;Abu-Ghazaleh,R;Kita,H;Sanderson,CJ;Gleich,GJ
通讯作者：
Gleich,GJ
纤连蛋白通过 VLA-4 和 VLA-5 整合素分子发出信号，促进初始 T 细胞和记忆 T 细胞的增殖。
影响因子：
4.4
作者：
Laurie S. Davis;N. Oppenheimer;J. L. Bednarczyk;Bradley W. McIntyre;P. E. Lipsky
通讯作者：
P. E. Lipsky
人嗜酸性粒细胞激活模式对豚鼠气管平滑肌收缩的影响。
DOI：
10.1152/ajplung.1993.264.5.l475
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Strek,ME;White,SR;Hsiue,TR;Kulp,GV;Williams,FS;Leff,AR
通讯作者：
Leff,AR
人类自然杀伤细胞表达 VLA-4 和 VLA-5，介导其与纤连蛋白的粘附。
影响因子：
4.4
作者：
A. Gismondi;Stefania Morrone;Martin J. Humphries;M. Piccoli;Luigi Frati;Angela Santoni
通讯作者：
Angela Santoni
影响因子：
14.2
作者：
FRIGAS, E;GLEICH, GJ
通讯作者：
GLEICH, GJ