Comparison of five methods for determination of total plasma protein concentration
Comparison of five methods for determination of total plasma protein concentration
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DOI：
10.1016/j.jbbm.2007.05.009
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发表时间：
2007-08-01
影响因子：
--
通讯作者：
Zihnioglu, Figen
中科院分区：
文献类型：
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作者：
Okutucu, Burcu;Dincer, Ayse;Zihnioglu, Figen
准确的总蛋白含量的定量通常是关键步骤，在一般生物化学研究和常规临床实验室实践的许多应用中都是常见的。在进行任何类型的蛋白质分析，特别是比较技术之前，准确定量样品中的蛋白质量是很重要的。为了评估总蛋白估计结果的质量，我们测试了五种方法，并应用于相同的血浆样本。为此，采用Bradford （comasassie Brilliant Blue）、Lowry （Folin-Ciocalteau）、Biuret、Pesce和Strande （Ponceau-S/TCA）和改进的Schaffner-Weismann （Amido Black 1013）方法。最后两种方法采用同时沉淀蛋白质与含酸染料溶液，然后溶解沉淀在NaOH溶液。结果表明，每种检测方法在灵敏度、易执行性、文献接受度、准确性和可重复性/变异系数方面都有优缺点。所有测试的方法都显示CV %
Quantitation of exact total protein content is often a key step and is common to many applications in general biochemistry research and routine clinical laboratory practice. Before embarking on any type of protein analysis, particularly comparative techniques, it is important to accurately quantitate the amount of protein in the sample. In order to assess the quality of total protein estimation results, five methods were tested and were applied to the same pooled plasma sample. For this aim, Bradford (Coomassie Brilliant Blue), Lowry (Folin-Ciocalteau), Biuret, Pesce and Strande (Ponceau-S/TCA), and modified method of Schaffner-Weismann (Amido Black 1013) were used. The last two methods employ simultaneous precipitation of proteins with the acid containing dye solutions followed by dissolution of precipitate in a NaOH solution. It is shown that each assay has advantages and disadvantages relative to sensitivity, ease of performance, acceptance in literature, accuracy and reproducibility/coefficient of variation. All of the methods tested show a CV %