Bacillus litorisediminis sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment
Bacillus litorisediminis sp. nov., a Thermophilic Bacterium Isolated from Mangrove Sediment
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DOI：
10.1007/s00284-023-03180-9
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发表时间：
2023-01
影响因子：
2.6
通讯作者：
R. Tang;Shang Yang;Shuang Han;Cheng-Jie Xie;G. Huang;Manik Prabhu Narsing Rao;Guo-Hong Liu;Shunhua Zhou
中科院分区：
文献类型：
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作者：
R. Tang;Shang Yang;Shuang Han;Cheng-Jie Xie;G. Huang;Manik Prabhu Narsing Rao;Guo-Hong Liu;Shunhua Zhou
从福建省张江口红树林国家级自然保护区沉积物中分离到两株需氧、革兰氏染色阳性、棒状、内孢子形成的嗜热菌株fjat -47801和FJAT-47835。在25-55°C（最适50°C）、pH 7.0 - 9.0（最适pH 7.0）、4.0% (w/v) NaCl（最适不加NaCl）条件下生长。菌株fjat - 47801t和FJAT-47835与嗜油芽孢杆菌16S rRNA基因序列相似性最高（98.5%）。FJAT-47801Tand FJAT-47835的16S rRNA基因序列相似性为99.9%，表明它们是同一物种。系统发育树（基于16S rRNA基因序列）和系统发育树（基于120个保守的细菌单拷贝基因）显示菌株fjat - 47801and FJAT-47835应该隶属于芽孢杆菌属。菌株fjat - 47801tm的甲基萘醌含量为MK-7。菌株fjat - 47801t的主要脂肪酸为iso-C15:0、anteiso-C15:0、iso-C17:0和C16:0。菌株fjat - 47801t的主要极性脂质是磷脂酰乙醇胺（PE）、二磷脂酰甘油（DPG）和磷脂酰甘油（PG）。菌株fjat - 47801t基因组DNA G+C含量为39.3%。菌株FJAT-47801TandB的平均核苷酸一致性（84.3%）和数字DNA-DNA杂交值（28.1%）。它们低于物种圈定的临界值。基于上述结果，菌株fjat -47801代表了芽孢杆菌属的一个新种，其名称为litorisdiminissp。建议11月11日。型应变为FJAT-47801T（=GDMCC 1.2712T= JCM 34875T）。
Two aerobic, Gram-staining-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-47801Tand FJAT-47835, were isolated from the sediment collected from Zhangjiang Estuary Mangrove National Nature Reserve in Fujian Province, China. Growth was observed at 25–55 °C (optimum, 50 °C) and pH 7.0–9.0 (optimum, pH 7.0), with up to 4.0% (w/v) NaCl (optimum, without NaCl). Strains FJAT-47801Tand FJAT-47835 showed the highest 16S rRNA gene sequence similarity toBacillus oleivorans(98.5%). The 16S rRNA gene sequence similarity between FJAT-47801Tand FJAT-47835 was 99.9% indicating they were the same species. Phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 120 conserved bacterial single-copy genes) trees showed that strains FJAT-47801Tand FJAT-47835 should be affiliated to the genusBacillus. The of menaquinone of strain FJAT-47801Twas MK-7. The major fatty acids of strain FJAT-47801Twere iso-C15:0, anteiso-C15:0, iso-C17:0, and C16:0. The major polar lipids strain FJAT-47801Twere phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), and phosphatidylglycerol (PG). The genomic DNA G+C content of strain FJAT-47801Twas 39.3%. The average nucleotide identity (84.3%) and the digital DNA–DNA hybridization value (28.1%) between strain FJAT-47801TandB. oleivoransCCTCC AB 2013353Twere below the cut-off level for species delineation. Based on the above results, strain FJAT-47801Trepresents a novel species of the genusBacillus, for which the nameBacillus litorisediminissp. nov., is proposed. The type strain is FJAT-47801T(=GDMCC 1.2712T= JCM 34875T).