Archaeal and bacterial community structures in the anoxic sediment of Antarctic meromictic lake Nurume-Ike
Archaeal and bacterial community structures in the anoxic sediment of Antarctic meromictic lake Nurume-Ike
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DOI：
10.1016/j.polar.2010.04.002
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发表时间：
2010-08
期刊：
影响因子：
1.8
通讯作者：
N. Kurosawa;Shota Sato;Y. Kawarabayasi;S. Imura;T. Naganuma
中科院分区：
文献类型：
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作者：
N. Kurosawa;Shota Sato;Y. Kawarabayasi;S. Imura;T. Naganuma
通过 16S rRNA 基因克隆的序列分析揭示了南极半交错湖 Nurume-Ike 缺氧沉积物中的原核生物群落结构。获得的古菌克隆（总共 205 个）仅由三个系统型组成，并且主要属于未培养的广古菌。具体来说，93% 的克隆被鉴定为海洋底栖 D 组古细菌系统型。与有限的古菌多样性相反，在 312 个细菌克隆中检测到了 53 种系统发育型。主要细菌系统型属于α-变形菌纲（占克隆的20%）、d-变形菌纲（9%）、浮霉菌目（7%）和蓝藻纲（7%）。还发现了少量属于γ-变形菌门、放线菌门、螺旋菌门、黄杆菌门和疣微菌门的克隆。总共 53% 的细菌克隆（由 13 种系统型组成）无法归类到任何已知的组中。这些结果表明，努鲁米-艾克湖沉积物的细菌群落由众多系统发育群组成，其多样性与之前报道的其他南极湖泊群落的多样性相当。然而有趣的是，努鲁姆艾克湖群落和西福尔德山地区的其他五个湖泊群落之间几乎没有共同的系统发育型。这是第一项对 500 多个 16S rDNA 克隆进行分析的综合研究，用于对南极湖泊沉积物样本进行微生物群落分析，其结果显着扩展了目前对南极湖泊细菌多样性的看法。
Prokaryotic community structures in the anoxic sediment of the Antarctic meromictic Lake Nurume-Ike were revealed by sequence analysis of 16S rRNA gene clones. The archaeal clones obtained (205 total) consisted of only three phylotypes, and were dominantly affiliated with uncultured euryarchaeotes. Specifically, 93% of the clones were identified as marine benthic group-D archaeal phylotype. In contrast to the limited archaeal diversity, 53 phylotypes were detected within 312 bacterial clones. Major bacterial phylotypes were affiliated with α-Proteobacteria (20% of clones), d-Proteobacteria (9%), Planctmycetales (7%), and Cyanobacteria (7%). A small numbers of clones belonging to γ-Proteobacteria, Actinobacteria, Spirochaetes, Flavobacteria, and Verrucomicrobia were also found. A total of 53% of the bacterial clones, consisting of 13 phylotypes, could not be classified into any known group. These results indicated that the bacterial community of Lake Nurume-Ike sediment consisted of numerous phylogenetic groups and had a diversity comparable to the diversity of other Antarctic lakes communities previously reported. Interestingly, however, there were very few phylotypes shared between the communities of lakes Nurume-Ike and five other lakes located in the Vestfold Hills area. This is the first comprehensive study to analyze more than 500 16S rDNA clones for microbial community analysis of an Antarctic lake sediment sample, and the results significantly expand current views of bacterial diversity in Antarctic lakes.