Cadmium mitigates ultraviolet-B stress in Anabaena doliolum: Enzymatic and non-enzymatic antioxidants
Cadmium mitigates ultraviolet-B stress in Anabaena doliolum: Enzymatic and non-enzymatic antioxidants
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DOI：
10.1007/s10535-007-0118-5
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发表时间：
2007-09
影响因子：
1.5
通讯作者：
P. Bhargava;N. Atri;A. Srivastava;L. Rai
中科院分区：
文献类型：
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作者：
P. Bhargava;N. Atri;A. Srivastava;L. Rai
单独和组合应用紫外线-B (UV-B) 和镉的影响，从氧释放、叶绿素 (Chl) 和蛋白质含量、脂质过氧化以及鱼腥藻的酶促和非酶抗氧化剂方面进行测量，结果表明 UV-B 诱导的氧化损伤比镉诱导的氧化损伤更大。虽然超氧化物歧化酶 (SOD) 受到 UV-B 的刺激大于 Cd，但过氧化氢酶 (CAT) 和谷胱甘肽还原酶 (GR) 的活性在 UV-B 处理后下降。然而，镉处理增强了抗坏血酸过氧化物酶 (APX) 和 GR 的活性。 CAT 活性在低剂量 Cd 时增加，但在高剂量 Cd 时下降。 UV-B 处理的细胞中类胡萝卜素 (Car) 含量的增加表明 Car 对 UV-B 应激具有屏蔽作用。在高剂量 Cd 和/或 UV-B 下，α-生育酚 (α-TOC) 含量增加 15 倍和 10 倍，证明了 α-TOC 的抗氧化作用。
Impact of ultraviolet-B (UV-B) and Cd, applied individually and in combination, measured in terms of oxygen-evolution, chlorophyll (Chl) and protein contents, lipid peroxidation, and enzymatic and non-enzymatic antioxidants ofAnabaena doliolum, revealed a greater oxidative damage induced by UV-B than by Cd. While superoxide dismutase (SOD) showed a greater stimulation by UV-B than Cd, the activities of catalase (CAT) and glutathione reductase (GR) declined after UV-B treatment. Cd treatment, however, enhanced the activities of ascorbate peroxidase (APX) and GR. CAT activity increased at low but decreased at high dose of Cd. Increase in carotenoid (Car) content in UV-B treated cells suggested a shielding effect of Car against UV-B stress. A 15-and 10-fold rise in α-tocopherol (α-TOC) content at high dose of Cd and/or UV-B offered testimony to the antioxidant role of α-TOC.