Cell cycle dependence of laminar determination in developing neocortex.
Cell cycle dependence of laminar determination in developing neocortex.
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发育新皮质层层决定的细胞周期依赖性。
DOI：
10.1126/science.254.5029.282
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Kaznowski,CE
中科院分区：
文献类型：
--
作者：
McConnell,SK;Kaznowski,CE
新皮质是在发育过程中按有序顺序产生的神经元层中形成图案的。这种细胞生日和板层命运之间的相关性促使人们研究发育中的皮质中神经元表型是如何决定的。在[~3H]胸腺嘧啶核苷标记后的不同时间，胚胎祖细胞被移植到较老的宿主脑中。移植神经元的层状命运与移植时它们的祖细胞在细胞周期中的位置有关。在S期移植的细胞的子代迁移到2/3层，宿主神经元也是如此。然而，在细胞周期较晚的时候移植的祖细胞产生的后代致力于它们正常的深层命运。因此，环境因素是层流命运的重要决定因素，但胚胎祖细胞对这种线索的反应能力经历了周期性的变化。
The neocortex is patterned in layers of neurons that are generated in an orderly sequence during development. This correlation between cell birthday and laminar fate prompted an examination of how neuronal phenotypes are determined in the developing cortex. At various times after labeling with [3H]thymidine, embryonic progenitor cells were transplanted into older host brains. The laminar fate of transplanted neurons correlates with the position of their progenitors in the cell cycle at the time of transplantation. Daughters of cells transplanted in S-phase migrate to layer 2/3, as do host neurons. Progenitors transplanted later in the cell cycle, however, produce daughters that are committed to their normal, deep-layer fates. Thus, environmental factors are important determinants of laminar fate, but embryonic progenitors undergo cyclical changes in their ability to respond to such cues.