Preparation of photoreactive phospholipid polymer nanoparticles to immobilize and release protein by photoirradiation
Preparation of photoreactive phospholipid polymer nanoparticles to immobilize and release protein by photoirradiation
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DOI：
10.1016/j.colsurfb.2015.07.073
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发表时间：
2015-11-01
影响因子：
5.8
通讯作者：
Ishihara, Kazuhiko
中科院分区：
文献类型：
--
作者：
Chen, Weixin;Inoue, Yuuki;Ishihara, Kazuhiko
制备了用于固定和释放蛋白质的光反应性和细胞相容性聚合物纳米颗粒。合成了一种水溶性两亲性磷脂聚合物聚（2-甲基丙烯酰氧乙基磷酰胆碱（MPC）-co-甲基丙烯酸正丁酯（BMA）-co-4-（4-（1-甲基丙烯酰氧乙基）-2-甲氧基-5-硝基苯氧基）丁酸（PL））（PMB-PL）。PMB-PL在PL单元处通过波长为365 nm的光照射进行裂解反应。此外，PMB-PL在水介质中使聚合物聚集，并作为乳化剂对可生物降解的聚乳酸（PLA）纳米颗粒进行表面改性。PMB-PL/PLA纳米粒子的形貌为球形，直径约为130 nm。PL单元中的羧酸基团可以通过共价键连接蛋白质。结合的蛋白质通过光诱导裂解反应释放。在60 s内，高达90%的固定化蛋白被光照射释放。从这些结果以及对MPC聚合物基本性质的理解，我们得出结论，PMB-PL/PLA纳米颗粒有可能用作智能载体，将蛋白质递送到生物系统，例如活细胞内部。(C)2015 Elsevier B. V.版权所有。
Photoreactive and cytocompatible polymer nanoparticles for immobilizing and releasing proteins were prepared. A water-soluble and amphiphilic phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)-co-4-(4-(1-methacryloyloxyethyl)-2-methoxy-5-nitrophenoxy) butyric acid (PL)) (PMB-PL) was synthesized. The PMB-PL underwent a cleavage reaction at the PL unit with photoirradiation at a wavelength of 365 nm. Additionally, the PMB-PL took polymer aggregate in aqueous medium and was used to modify the surface of biodegradable poly(L-lactic acid) (PLA) nanoparticle as an emulsifier. The morphology of the PMB-PL/PLA nanoparticle was spherical and approximately 130 nm in diameter. The carboxylic acid group in the PL unit could immobilize proteins by covalent bonding. The bound proteins were released by a photoinduced cleavage reaction. Within 60s, up to 90% of the immobilized proteins was released by photoirradiation. From these results and with an understanding of the fundamental properties of MPC polymers, we concluded that PMB-PL/PLA nanoparticles have the potential to be used as smart carriers to deliver proteins to biological systems, such as the inside of living cells. (C) 2015 Elsevier B.V. All rights reserved.