A Porphyromonas gingivalis genetic locus encoding a heme transport system.
A Porphyromonas gingivalis genetic locus encoding a heme transport system.
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DOI：
10.1034/j.1399-302x.2000.150609.x
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发表时间：
2000-12
影响因子：
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通讯作者：
N. Slakeski;S. Dashper;P. Cook;C. Poon;C. Moore;E. Reynolds
中科院分区：
文献类型：
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作者：
N. Slakeski;S. Dashper;P. Cook;C. Poon;C. Moore;E. Reynolds
牙龈卟啉单胞菌与牙周炎的发病和进展有关，而血红素用于体外生长的可用性与动物模型中细菌的毒力有关。我们在此报告牙龈卟啉单胞菌 TonB 连接的外膜受体基因 tlr 的克隆和序列分析。该基因先前被鉴定为 TonB 连接的粘附素基因 tla，并被证明对于低浓度氯化血红素的生长至关重要。 tlr 基因紧邻四个开放阅读框 (htrABCD) 的下游，这些开放阅读框编码假定的 ATP 结合盒转运系统，其序列与其他细菌的血红素转运系统相似。对牙龈卟啉单胞菌 W50 mRNA 的分析表明，htrABCD 基因的共转录与其他细菌的氯高铁血红素转运基因类似。
Porphyromonas gingivalis has been implicated in the onset and progression of periodontitis and the availability of hemin for in vitro growth has been associated with virulence of the bacterium in animal models. We report here the cloning and sequence analysis of a P. gingivalis TonB-linked outer membrane receptor gene tlr. This gene was previously identified as a TonB-linked adhesin gene tla and shown to be essential for growth at low concentrations of hemin. The tlr gene is immediately downstream of four open reading frames (htrABCD) that encode a putative ATP binding cassette transport system with sequence similarlity to heme transport systems of other bacteria. Analysis of P. gingivalis W50 mRNA revealed that the htrABCD genes are cotranscribed similar to hemin transport genes of other bacteria.