Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride
Antioxidant Activity of Extract and Its Major Constituents from Okra Seed on Rat Hepatocytes Injured by Carbon Tetrachloride
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秋葵籽提取物及其主要成分对四氯化碳损伤大鼠肝细胞的抗氧化活性
DOI：
10.1155/2014/341291
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Tang, Zhaoxin
中科院分区：
文献类型：
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作者：
Hu, Lianmei;Yu, Wenlan;Tang, Zhaoxin
研究了黄秋葵种子总酚提取物（TPE）及其主要成分对四氯化碳（CCl 4）诱导的大鼠肝细胞氧化应激的抗氧化作用和保护作用。主要酚类化合物为槲皮素3-O-葡萄糖基（1 -> 6）葡萄糖苷（QDG）和槲皮素3-O-葡萄糖苷（QG）。黄秋葵种子中的TPE、QG和QDG表现出优异的还原能力和自由基清除能力，包括α，α-二苯基-β-苦基肼基（DPPH）、超氧阴离子和羟基自由基。总的来说，QG和QDG清除DPPH自由基的能力和还原力均高于TPE，清除超氧阴离子自由基和羟自由基的能力高于QDG。TPE、QG和QDG预处理可显著减轻CCl 4对大鼠肝细胞的毒性，减轻脂质过氧化反应，提高SOD和CAT活性，降低GPT和GOT活性。TPE和QG对大鼠肝细胞的保护作用强于QDG。然而，四氯化碳对大鼠肝细胞的细胞毒性不受TPE，QG，QDG后处理。提示TPE、QG和QDG对大鼠肝细胞抗氧化损伤的保护作用与其直接抗氧化能力和诱导的抗氧化酶活性有关。
The antioxidant activities and protective effects of total phenolic extracts (TPE) and their major components from okra seeds on oxidative stress induced by carbon tetrachloride (CCl4) in rat hepatocyte cell line were investigated. The major phenolic compounds were identified as quercetin 3-O-glucosyl (1 -> 6) glucoside (QDG) and quercetin 3-O-glucoside (QG). TPE, QG, and QDG from okra seeds exhibited excellent reducing power and free radical scavenging capabilities including alpha, alpha-diphenyl-beta-picrylhydrazyl (DPPH), superoxide anions, and hydroxyl radical. Overall, DPPH radical scavenging activity and reducing power of QG and QDG were higher than those of TPE while superoxide and hydroxyl radical scavenging activities of QG and TPE were higher than those of QDG. Furthermore, TPE, QG, and QDG pretreatments significantly alleviated the cytotoxicity of CCl4 on rat hepatocytes, with attenuated lipid peroxidation, increased SOD and CAT activities, and decreased GPT and GOT activities. The protective effects of TPE and QG on rat hepatocytes were stronger than those of QDG. However, the cytotoxicity of CCl4 on rat hepatocytes was not affected by TPE, QG, and QDG posttreatments. It was suggested that the protective effects of TPE, QG, and QDG on rat hepatocyte against oxidative stress were related to the direct antioxidant capabilities and the induced antioxidant enzymes activities.