IDENTIFICATION OF A 3RD REGION OF CELL-SPECIFIC ALTERNATIVE SPLICING IN HUMAN FIBRONECTIN MESSENGER-RNA
IDENTIFICATION OF A 3RD REGION OF CELL-SPECIFIC ALTERNATIVE SPLICING IN HUMAN FIBRONECTIN MESSENGER-RNA
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DOI：
10.1073/pnas.84.20.7179
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
KORNBLIHTT, AR
中科院分区：
文献类型：
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作者：
GUTMAN, A;KORNBLIHTT, AR
我们在这里描述了人类纤维连接蛋白(FN)的第三个可变区，这是由于选择性RNA剪接造成的。先前已经报道了另外两个选择性剪接位置(ED和IIICS)。第三个区域涉及一个273个核苷酸的外显子，编码一个91个氨基酸的III型同源重复序列，位于FN的DNA-和细胞结合域之间，它要么包含在FN mRNA中，要么排除在FN信使核糖核酸之外。由外显子跳跃机制产生的两种mRNA变体存在于已知的合成FN细胞形式的细胞中。然而，作为血浆FN来源的肝细胞只产生信使，没有额外的III型序列。因此，这里描述的区域在结构和功能上都类似于前面描述的ED(额外结构域)区域，位于分子的C末端，在细胞和肝素(HEP 2)结合域之间。我们得出结论，额外的III型重复序列(命名为EDII)和ED都代表仅限于细胞FN的序列。结合目前所描述的三个区域的所有可能的剪接模式，可以从单个基因产生多达20个不同的FN多肽。
We describe here a third region of variability in human fibronectin (FN) due to alternative RNA splicing. Two other positions of alternative splicing have been reported previously (ED and IIICS). The third region involves a 273-nucleotide exon encoding exactly one 91-amino acid repeat of type III homology, located between the DNA- and the cell-binding domains of FN, which is either included in or excluded from FN mRNA. The two mRNA variants arising by an exon-skipping mechanism are present in cells known to synthesize the cellular form of FN. However, liver cells, which are the source of plasma FN, produce only messengers without the extra type III sequence. Therefore, the region described here resembles, both structurally and functionally, the previously described ED (for extra domain) region, located toward the C terminus of the molecule, between the cell- and heparin- (hep 2) binding domains. We conclude that both the extra type III repeat (named EDII) and ED represent sequences restricted to cellular FN. Combination of all the possible patterns of splicing in the three regions described to date may generate up to 20 distinct FN polypeptides from a single gene.