Long-range population dynamics of anatomically defined neocortical networks
Long-range population dynamics of anatomically defined neocortical networks
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DOI：
10.7554/elife.14679
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发表时间：
2016-05-24
期刊：
影响因子：
7.7
通讯作者：
Helmchen, Fritjof
中科院分区：
文献类型：
--
作者：
Chen, Jerry L.;Voigt, Fabian F.;Helmchen, Fritjof
新皮层区域活动的协调对于哺乳动物的大脑功能至关重要。要了解这一过程，需要同时测量整个皮层的功能。为了将直接的皮质-皮质相互作用与神经元相关性的其他来源分离，还期望将跨区域记录靶向于解剖学上在区域之间投射的神经元亚群。在这里，我们结合了解剖示踪剂与一种新的多区域双光子显微镜进行同步钙成像小鼠初级（S1）和次级（S2）体感须皮层在纹理辨别行为，特别是识别前馈和反馈神经元。我们发现，协调S1-S2活动增加运动行为，如目标导向的搅拌和舔。这种效应并不特异于识别的前馈和反馈神经元。然而，这些相互投射的神经元尤其参与了区域间协调，当运动行为与胡须纹理触摸配对时，这表明直接S1-S2相互作用是依赖于感觉的。我们的研究结果表明，特定的功能协调的解剖学上确定的投射神经元跨感觉皮层。
The coordination of activity across neocortical areas is essential for mammalian brain function. Understanding this process requires simultaneous functional measurements across the cortex. In order to dissociate direct cortico-cortical interactions from other sources of neuronal correlations, it is furthermore desirable to target cross-areal recordings to neuronal subpopulations that anatomically project between areas. Here, we combined anatomical tracers with a novel multiarea two-photon microscope to perform simultaneous calcium imaging across mouse primary (S1) and secondary (S2) somatosensory whisker cortex during texture discrimination behavior, specifically identifying feedforward and feedback neurons. We find that coordination of S1-S2 activity increases during motor behaviors such as goal-directed whisking and licking. This effect was not specific to identified feedforward and feedback neurons. However, these mutually projecting neurons especially participated in inter-areal coordination when motor behavior was paired with whisker-texture touches, suggesting that direct S1-S2 interactions are sensory-dependent. Our results demonstrate specific functional coordination of anatomically-identified projection neurons across sensory cortices.