Formation of Charcot-Leyden crystals by human basophils.
Formation of Charcot-Leyden crystals by human basophils.
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DOI：
10.1084/jem.155.6.1597
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发表时间：
1982-06-01
期刊：
影响因子：
--
通讯作者：
Gleich GJ
中科院分区：
文献类型：
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作者：
Ackerman SJ;Weil GJ;Gleich GJ
目前认为夏科-莱登晶体 (CLC) 是嗜酸性粒细胞所特有的，是活跃的嗜酸性粒细胞炎症或增殖的标志。 1965 年，Archer 和 Blackwood 对 CLC 与嗜酸性粒细胞的独特性提出了质疑 (9)，但他们对嗜碱性粒细胞中 CLC 形成的论证被忽视，后来被驳回 (1)，认为这是富含嗜碱性粒细胞的细胞悬液中嗜酸性粒细胞污染的结果。我们重新审视了这个问题，发现从正常个体的外周血中获得的嗜碱性粒细胞形成了 CLC，并且嗜碱性粒细胞含有一种在免疫化学上与嗜酸性粒细胞 CLC 蛋白无法区分的蛋白质。这些结论基于以下发现：(a) 通过对高度富集的嗜碱性粒细胞悬液中含晶体的细胞进行特异性组织化学染色来证明嗜碱性粒细胞中的晶体形成，所述悬浮液是通过荧光激活细胞分选仪 (FACS) 纯化表面 IgE 阳性细胞而制备的，(b) 通过 FACS 对表面 IgE 阳性细胞（主要是嗜碱性粒细胞）的富集也对染色阳性的细胞进行了富集 嗜酸性粒细胞 CLC 蛋白的免疫荧光，以及 (c) 通过放射免疫测定法在由含有 97-99% 嗜碱性粒细胞且绝对不含污染性嗜酸性粒细胞的纯化嗜碱性粒细胞悬浮液制备的细胞提取物中测量 CLC 蛋白。这些嗜碱性粒细胞提取物含有免疫化学上与嗜酸性粒细胞 CLC 蛋白无法区分的蛋白质。基于这些发现，CLC 或包含晶体的蛋白质（溶血磷脂酶）不再被认为是嗜酸性粒细胞所特有的。我们现在必须考虑这样一种可能性：组织、痰或粪便中存在 CLC 也可能代表嗜碱性粒细胞参与疾病过程。
Charcot-Leyden crystals (CLC) are currently believed to be unique to the eosinophil and a hallmark of active eosinophilic inflammation or proliferation. The distinctiveness of the CLC to the eosinophil was questioned in 1965 by Archer and Blackwood (9), but their demonstration of CLC formation in basophils was ignored and later dismissed (1) as being the result of eosinophil contamination of basophil-enriched cell suspensions. We reexamined this question and showed that basophils obtained from the peripheral blood of normal individuals form CLC and that basophils contain a protein that is immunochemically indistinguishable from eosinophil CLC protein. These conclusions are based upon the findings that (a) crystal formation in basophils was demonstrated by specific histochemical staining of crystal-containing cells in highly enriched basophil suspensions prepared by fluorescence- activated cell sorter (FACS) purification of surface IgE-positive cells, (b) that enrichment for surface IgE-positive cells (primarily basophils) by the FACS also enriched for cells staining positively by immunofluorescence for eosinophil CLC protein, and (c) that CLC protein was measured by radioimmunoassay in cell extracts prepared from purified basophil suspensions containing 97-99% basophils and absolutely no contaminating eosinophils. These basophil extracts contained a protein immunochemically indistinguishable from eosinophil CLC protein. Based upon these findings, the CLC or the protein comprising the crystal (lysophospholipase) can no longer be considered as distinctive to the eosinophil. We must now consider the possibility that the presence of CLC in tissues, sputum, or stool may also represent basophil involvement in disease processes.