Somatic cell genetic approaches to Down's syndrome.
Somatic cell genetic approaches to Down's syndrome.
复制标题
唐氏综合症的体细胞遗传学方法。
DOI：
10.1111/j.1749-6632.1982.tb26844.x
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发表时间：
1982
影响因子：
5.2
通讯作者：
Graw,S
中科院分区：
文献类型：
--
作者：
Patterson,D;Jones,C;Scoggin,C;Miller,YE;Graw,S
对嘌呤合成缺陷的中国仓鼠卵巢细胞突变体和这些突变体与人细胞杂交的体细胞遗传学分析进行了描述。数据证实，嘌呤合成酶，AdeC和AdeG的两个基因，可以协调调节哺乳动物细胞。人-仓鼠杂交细胞Ade C/21含有仓鼠染色体的正常补体和人21号染色体作为其通过电泳和免疫遗传学技术可识别的唯一人类遗传组分，对该细胞的分析表明，可以在这些杂交细胞中检测到与人21号染色体的存在相关的基因，以及合成特异性多肽和特异性人致死细胞表面抗原所需的基因。
Somatic cell genetic analysis of mutants of Chinese hamster ovary cells with deficient purine synthesis and of hybrids between these mutants and human cells is described. Data are presented substantiating that two genes for enzymes of purine synthesis, AdeC and AdeG, can be coordinately regulated in mammalian cells. Analysis of a human-hamster hybrid cell, Ade C/21, which contains a normal complement of hamster chromosomes and human chromosome 21 as its only human genetic component recognizable by electrophoretic and immunogenetic techniques demonstrates that genes associated with the presence of human chromosome 21 and required for the synthesis of specific polypeptides and specific human lethal cell surface antigens can be detected in these hybrids.