FLUORESCENCE DECAY AND QUANTUM YIELD CHARACTERISTICS OF ACRIDINE-ORANGE AND PROFLAVINE BOUND TO DNA
FLUORESCENCE DECAY AND QUANTUM YIELD CHARACTERISTICS OF ACRIDINE-ORANGE AND PROFLAVINE BOUND TO DNA
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DOI：
10.1016/0301-4622(77)85009-6
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发表时间：
1977-01-01
影响因子：
3.8
通讯作者：
STEINER, RF
中科院分区：
文献类型：
--
作者：
KUBOTA, Y;STEINER, RF
研究了与DNA结合的吖啶橙和前列腺素的荧光性质(量子产率、衰减曲线、寿命和偏振)与核苷酸/染料(P/D)的关系。通过相移测量确定了平均荧光寿命。吖啶橙的寿命和量子产率随着P/D比的增加而平行增加。前黄素的寿命与量子产率之间没有平行关系，寿命在P/D=10附近出现最小值。然后用单光子计数技术测量了荧光衰减曲线，并借助于矩量法和拉普拉斯变换法进行了分析。结合的吖啶橙的荧光衰减在P/D=10以上可能是指数衰减，结合的前黄素在P/D=100以上是指数衰减，但随着P/D比的减小而明显偏离指数。荧光偏振结果表明，这种现象是结合在荧光中心(AT对)和猝灭中心(GC对)上的前黄素分子之间Forster能量转移的结果。结合的前黄素和吖啶橙的临界转移距离分别为26.4和37.0。
Fluorescence properties (quantum yield, decay curve, lifetime and polarization) of acridine orange and proflavine bound to DNA were examined as a function of nucleotide to dye (P/D) ratio. Mean fluorescence lifetimes were determined by the phase-shift measurements. The lifetime and quantum yield of acridine orange increased in a parallel fashion with increasing P/D ratio. There was no parallel relation between the lifetime and quantum yield for proflavine; the lifetime showed a minimum around P/D = 10. Fluorescence decay curves were then measured by the monophoton counting technique and analyzed with the aid of the method of moments and the Laplace transform method. The fluorescence decay of bound acridine orange was probably exponential above P/D = 10. The decay of bound proflavine was exponential above P/D = 100, but markedly deviated from exponentiality with decreasing P/D ratio. The results of fluorescence polarization suggested that this phenomenon is the result of Forster energy transfer between proflavine molecules bound to the fluorescent site (AT pair) and bound to the quenching site (GC pair). Critical transfer distances were 26.4 and 37.0 .ANG., respectively, for bound proflavine and acridine orange.