Transcriptome profiling reveals major structural genes, transcription factors and biosynthetic pathways involved in leaf senescence and nitrogen remobilization in rainfed spring wheat under different nitrogen fertilization rates
Transcriptome profiling reveals major structural genes, transcription factors and biosynthetic pathways involved in leaf senescence and nitrogen remobilization in rainfed spring wheat under different nitrogen fertilization rates
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DOI：
10.1016/j.ygeno.2022.110271
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发表时间：
2022-01-22
期刊：
影响因子：
4.4
通讯作者：
Zeng, Min
中科院分区：
文献类型：
--
作者：
Effah, Zechariah;Li, Lingling;Zeng, Min
本研究分析了不同施氮量（N）下雨养春小麦品种开花至灌浆末期旗叶转录变化：0 kg/ha（NN）、52.5 kg/ha（LN）和210 kg/ha（HN）/ha（HN）。LN和HN中分别检测到4485和4627个差异表达基因。氮素的不同施用改变了植物激素信号转导、植物有丝分裂原活化蛋白激酶信号转导、光合作用、苯丙烷类化合物生物合成和ATP结合盒转运蛋白等途径。茉莉酸、脱落酸、水杨酸和油菜素内酯相关基因促进NN和LN叶片衰老，而生长素、赤霉素酸和细胞分裂素基因抑制HN叶片衰老。主要转录因子：生长素/吲哚乙酸（AUX/IAA）、无顶端分生组织（NAC）和WRKY在HN和LN中的表达均高于NN。DEG、途径和转录因子为调控小麦叶片衰老和氮素再利用提供了有价值的见解。
The present study was undertaken to profile transcriptional changes in flag leaves between anthesis and end of grain filling stages of rainfed spring wheat cultivar under varying nitrogen (N) application rates: 0 kg/ha (NN), 52.5 kg/ha (LN), and 210 kg/ha (HN). A total of 4485 and 4627 differentially expressed genes (DEGs) were detected in LN and HN, respectively. The differential application of N altered several pathways; including plant hormone signal transduction, mitogen-activated protein kinase signaling pathway-plant, photosynthesis, phe-nylpropanoid biosynthesis and ATP-binding cassette transporters. Jasmonic acid, abscisic acid, salicylic acid and brassinosteroid related genes promoted leaf senescence in NN or LN, whereas auxin, gibberellin acid and cyto-kinins genes inhibited leaf senescence in HN. Major transcription factors: auxin/indole-3-acetic acid (AUX/IAA), no apical meristem (NAC) and WRKY expressed higher in either HN or LN than NN. The DEGs, pathways and transcription factors provide valuable insight for manipulation of leaf senescence and N remobilization in wheat.