Association mapping for seed size and shape traits in soybean cultivars
Association mapping for seed size and shape traits in soybean cultivars
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大豆品种种子大小和形状性状的关联作图
DOI：
10.1007/s11032-012-9833-5
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发表时间：
2013-04-01
影响因子：
3.1
通讯作者：
Zhang, Yuan-Ming
中科院分区：
文献类型：
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作者：
Niu, Yuan;Xu, Yu;Zhang, Yuan-Ming
大豆（Glycine maxL.）种子的大小和形状性状与种子产量和外观品质密切相关。以往的研究主要来自于双亲本分离群体，对大豆品种的结果知之甚少。本研究利用分层随机抽样从中国6个地理生态型中获得的257个大豆品种，利用135个简单序列重复标记的信息和经验贝叶斯算法实现的上位关联作图方法，对这些性状进行了关联作图。2008-2010年，通过种子长度（SL）、宽度（SW）和厚度（ST）测量种子大小，通过种子长宽比（SLW）、长厚比（SLT）和宽厚比（SWT）评价种子形状。共鉴定出59个主效QTL位点和31个QTL-环境互作位点。其中25个QTL与至少两个性状同时相关；80个QTL（90%）经浓缩压缩混合线性模型分析证实；除qsl -5、qSW-7e、qst -5-2和qslw -2外，大部分QTL的大小较小。根据检测到的QTL的等位基因效应估计，可以挖掘出优质等位基因：例如，与satt453连锁的QTL的307 bp等位基因是种子长度最好的等位基因。这些精英等位基因可用于设计亲本组合；例如，“正和zhibanzi”与“南农大黄豆”杂交可提高种子长度，“林豆10”、“德青香竹豆”、“宁海下之豆”、“正和zhibanzi”、0803、商秋7605和0831组合可金字塔化42个精英等位基因。
Seed size and shape traits are closely related to seed yield and appearance quality in soybean (Glycine maxL.). Previous studies were mainly derived from bi-parental segregating populations and relatively little is known about the results in soybean cultivars. In this study, 257 soybean cultivars obtained by stratified random sampling from six geographic ecotypes in China were used to carry out association mapping for these traits using information from 135 simple sequence repeat markers and an epistatic association mapping approach implemented using an empirical Bayes algorithm. In this analysis, seed size was measured by seed length (SL), width (SW) and thickness (ST), and seed shape was evaluated by seed length-to-width (SLW), length-to-thickness (SLT) and width-to-thickness (SWT) ratios, in 2008–2010. A total of 59 main-effect quantitative trait loci (QTL) and 31 QTL-by-environment interactions were identified. Among them, 25 QTL were associated simultaneously with at least two traits; 80 QTL (90 %) could be confirmed by enriched compression mixed linear model analysis; and the size for a large number of detected QTL was minor, except forqSL-5,qSW-7e,qST-5-2andqSLW-2. According to the estimates for the allelic effects of the detected QTL, elite alleles could be mined: for example, the 307-bp allele of QTL linked to satt453 was the best for seed length. These elite alleles could be used to design parental combinations; e.g., the cross of Zhenghezhibanzi and Nannongdahuangdou might improve seed length, and the combination of cultivars Lindou10, Deqingxiangzhudou, Ninghaixiazhidou, Zhenghezhibanzi, 0803, Shangqiu7605 and 0831 might pyramid 42 elite alleles.