Using genome resequencing to investigate racial structure, genetic diversity, sexual reproduction and hybridisation in the pine pathogen Dothistroma septosporum
Using genome resequencing to investigate racial structure, genetic diversity, sexual reproduction and hybridisation in the pine pathogen Dothistroma septosporum
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DOI：
10.1016/j.funeco.2020.100921
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发表时间：
2020-06-01
期刊：
影响因子：
2.9
通讯作者：
Hoebe, Peter N.
中科院分区：
文献类型：
--
作者：
Ennos, Richard A.;Sjokvist, Elisabet Ingrid;Hoebe, Peter N.
全基因组重测序的25个菌株的子囊菌松病原体Dothistroma septosporum揭示了存在三个遗传上不同的种族在苏格兰，确认以前的结果的基础上微卫星标记。来自苏格兰（NPR）的遗传一致的土著种族与来自英格兰和法国的遗传多样性的南方种族（SR）密切相关。种族LPR，仅限于小屋杆松，是从这些遗传上的分歧，但与加拿大（CAN）分离物有密切的亲和力。CAN和SR种族内的个人之间的基因组比较发现短而分散的区域附近的序列同一性，表明显着的有性生殖。LPR内的序列同一性模式表明，它来自于从北美引进的单一有性子实体。最后，确定了土著NPR和引入SR之间的第一代种族间杂交，标志着D的进化潜力增加。septosporum在苏格兰，和本地苏格兰松（樟子松）的风险增加。(C)2020 Elsevier Ltd和英国真菌学会。All rights reserved.
Whole genome resequencing of 25 isolates of the ascomycete pine pathogen Dothistroma septosporum revealed the presence of three genetically distinct races in Scotland, confirming previous results based on microsatellite markers. The genetically uniform indigenous race from Scotland (NPR), is closely related to an introduced and genetically diverse southern race (SR) from England and France. Race LPR, confined to lodge-pole pine, is genetically divergent from these, but has close affinity with Canadian (CAN) isolates. Genome comparisons between individuals within CAN and SR races revealed short and scattered regions of near sequence identity, indicating significant sexual reproduction. Sequence identity patterns within LPR suggest that it derives from introduction of a single sexual fruit body from North America. Finally a first generation interracial hybrid between indigenous NPR and introduced SR was identified, signalling an increase in the evolutionary potential of D. septosporum in Scotland, and an enhanced risk to native Scots pine (Pinus sylvestris). (C) 2020 Elsevier Ltd and British Mycological Society. All rights reserved.