Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)
Cloning and characterization of the drought-resistance OsRCI2-5 gene in rice (Oryza sativa L.)
复制标题
水稻抗旱OsRCI2-5基因的克隆与鉴定（Oryza sativa L.）
DOI：
10.4238/2014.may.23.13
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Deng, H. F.
中科院分区：
文献类型：
--
作者：
Li, L.;Li, N.;Deng, H. F.
利用水稻基因组芯片技术，检测了超级杂交稻两优培九母本培矮64 S在低温、干旱和高温胁迫下不同发育时期不同组织的基因组表达谱，筛选表达上调和下调的基因。在本研究中，我们筛选了抗旱基因OsRCI 2 -5，之后构建了组成型OsRCI 2 -5构建体并将其转入日本晴。在PEG-6000和干旱处理后，我们发现OsRCI 2 -5基因提高了日本晴的抗旱性。基因表达谱分析表明，OsRCI 2 -5基因在水稻叶片、茎和花器官中均有表达。亚细胞定位结果表明，该基因位于细胞膜上，因此，我们可以推断，膜信号肽负责信号转导。
The genomic expression profile of the super-hybrid rice Liangyoupeijiu female parent Pei'ai 64S in different tissues at different developmental stages under low temperature, drought, and high temperature stresses were detected using an Affymetrix GeneChip Rice Genome Array to screen upregulated and downregulated genes. In this study, we screened the drought-resistant gene OsRCI2-5, after which a constitutive OsRCI2-5 construct was created and transferred into Nipponbare. After polyethylene glycol-6000 and drought treatment, we found that the OsRCI2-5 gene improved the drought resistance of Nipponbare. Gene expression profiling showed that the OsRCI2-5 gene was expressed in the rice leaves, stems, and flower organs. Subcellular localization revealed that the gene was located in the membranes, and hence, we can deduce that a membrane signal peptide was responsible for signal transduction.