Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans
Mutation in a mitochondrial ribosomal protein causes increased sensitivity to oxygen with decreased longevity in the nematode Caenorhabditis elegans
复制标题
线粒体核糖体蛋白突变导致线虫对氧的敏感性增加，寿命缩短
DOI：
10.1111/j.1365-2443.2010.01469.x
复制
发表时间：
2011
期刊：
影响因子：
2.1
通讯作者：
Ayusawa D.
中科院分区：
文献类型：
--
作者：
Fujii M;Shikatani K;Ogura K;Goshima Y;Ayusawa D.
氧气对动物来说是必不可少的，但高浓度的氧气对它们是有毒的，这可能是因为活性氧（ROS）的增加。许多基因参与了ROS产生的反应，但没有太多的注意力集中在他们身上。为了鉴定这些基因，我们在线虫秀丽隐杆线虫中筛选了对氧化应激敏感性改变的突变体，并分离出一个突变体oxy-5（qa 5002）。oxy-5显示出对氧的敏感性增加，寿命缩短。inoxy-5的寿命缩短可能是由于氧化应激增加，因为当在缺氧条件下培养时，它会恢复到正常水平。我们的遗传分析显示，负责oxy-5的基因编码一种类似于线粒体核糖体蛋白S36的蛋白质。OXY-5蛋白在神经元、咽和肠中高度表达，并且来自泛神经元H20启动子的oxy-5表达有效地抑制了对氧inoxy-5的敏感性增加。这些结果表明，oxy-5在调节秀丽隐杆线虫神经细胞对氧的敏感性中起重要作用。
Oxygen is essential for animals, but high concentrations of oxygen are toxic to them probably because of an increase in reactive oxygen species (ROS). Many genes are involved in the reactions from which ROS are generated, but not much attention has been focused on them. To identify these genes, we screened for mutants with an altered sensitivity to oxidative stress in the nematodeCaenorhabditis elegansand isolated a mutant,oxy‐5(qa5002).oxy‐5showed an increased sensitivity to oxygen and decreased longevity. The decreased life span inoxy‐5was probably due to increased oxidative stress because it was recovered to a normal level whenoxy‐5was cultured under hypoxic conditions. Our genetic analysis has revealed that the responsible gene foroxy‐5encodes a protein similar to mitochondrial ribosomal protein S36. The OXY‐5 protein was highly expressed in the neurons, pharynx, and intestine, and expression ofoxy‐5from the pan‐neuronalH20promoter efficiently suppressed the increased sensitivity to oxygen inoxy‐5. These findings suggested thatoxy‐5played an important role in the regulation of the sensitivity to oxygen in neuronal cells inC. elegans.