Oxidative stress, free radicals and protein peroxides
Oxidative stress, free radicals and protein peroxides
复制标题
DOI：
10.1016/j.abb.2015.10.021
复制
发表时间：
2016-04-02
影响因子：
3.9
通讯作者：
Gebicki, Janusz M.
中科院分区：
文献类型：
--
作者：
Gebicki, Janusz M.
在细胞和组织中氧化应激下产生的初级自由基产生反应性次级自由基的级联，其以由反应速率常数和靶浓度确定的效率攻击生物分子。蛋白质是突出的靶点，因为它们构成细胞和组织的大部分有机内容物，并且容易与许多次级自由基反应。这些反应通常导致碳中心自由基的形成，其通常在体内转化为过氧自由基并最终转化为半稳定的氢过氧化物。所有这些中间体都可以引发生物损伤。本文概述了电离辐射应用于自由基研究的优点，特别是涉及所需自由基的产生，其反应动力学的研究和与生物系统中的事件的相关结果。在一个这样的应用中，蛋白质氢过氧化物在辐照细胞中的形成被细胞内抗坏血酸盐和谷胱甘肽抑制。(C)2015 Elsevier Inc. All rights reserved.
Primary free radicals generated under oxidative stress in cells and tissues produce a cascade of reactive secondary radicals, which attack biomolecules with efficiency determined by the reaction rate constants and target concentration. Proteins are prominent targets because they constitute the bulk of the organic content of cells and tissues and react readily with many of the secondary radicals. The reactions commonly lead to the formation of carbon-centered radicals, which generally convert in vivo to peroxyl radicals and finally to semistable hydroperoxides. All of these intermediates can initiate biological damage. This article outlines the advantages of the application of ionizing radiations to studies of radicals, with particular reference to the generation of desired radicals, studies of the kinetics of their reactions and correlating the results with events in biological systems. In one such application, formation of protein hydroperoxides in irradiated cells was inhibited by the intracellular ascorbate and glutathione. (C) 2015 Elsevier Inc. All rights reserved.