Single-walled carbon nanotubes/DNA hybrids in water are highly stable
Single-walled carbon nanotubes/DNA hybrids in water are highly stable
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DOI：
10.1016/j.cplett.2008.02.089
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发表时间：
2008-04-10
影响因子：
2.8
通讯作者：
Nakashima, Naotoshi
中科院分区：
文献类型：
--
作者：
Noguchi, Yuichi;Fujigaya, Tsuyohiko;Nakashima, Naotoshi
使用尺寸排阻色谱法（SEC）从dsDNA溶解的单壁碳纳米管（SWNT）的水溶液（溶液1）中除去未结合的双链DNA（dsDNA）。溶液1的SEC色谱图显示两个分离的峰，并且较早的洗脱液级分被分离成四个尺寸分离的级分fr 1-fr 4，其中发现存在单独溶解的SWNT。通过重新注射fr 1-fr 4评价DNA/SWNT杂合体的稳定性。结果表明，fr 1-fr 4的色谱图在保存1个月后仍与原材料的色谱图一致，表明dsDNA/SWNT杂交体在水中具有很高的稳定性。(C)2008 Elsevier B. V.保留所有权利。
Unbound double-stranded DNA (dsDNA) was removed from an aqueous solution (solution 1) of dsDNA-solubilized single-walled carbon nanotubes (SWNTs) using size-exclusion chromatography (SEC). The SEC chromatogram of solution 1 showed two separated peaks, and the earlier eluent fraction was separated into four size-separated fractions, fr1-fr4, in which individually solubilized SWNTs were found to exist. The stability of the DNA/SWNT hybrids was evaluated by the re-injection of fr1-fr4. It was found that the chromatograms of fr1-fr4 were identical to those of the original ones even after storage for one month, indicating the high stability of the dsDNA/SWNT hybrids in water. (C) 2008 Elsevier B.V. All rights reserved.