Identification of a novel spore wall protein (SWP26) from microsporidia Nosema bombycis
Identification of a novel spore wall protein (SWP26) from microsporidia Nosema bombycis
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家蚕微孢子虫中新型孢子壁蛋白 (SWP26) 的鉴定
DOI：
10.1016/j.ijpara.2008.08.011
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发表时间：
2009-03-01
影响因子：
4
通讯作者：
Zhou, Zeyang
中科院分区：
文献类型：
--
作者：
Li, Yanhong;Wu, Zhengli;Zhou, Zeyang
微孢子虫是一种专性的细胞内寄生虫，与真菌有关，具有抵抗各种环境胁迫的孢子。这些生物的刚性孢子壁由两个主要层组成，即外孢子和内生孢子。两种孢子壁蛋白（孢子内壁蛋白-SWP 30和孢子外壁蛋白-SWP 32）已在家蚕微孢子虫中鉴定。在这项研究中，使用MALDI-TOF-MS技术，我们已经确定了一个新的25.7 kDa的孢子壁蛋白（SWP 26）的单克隆抗体2G 10识别。SWP 26被预测具有信号肽、四个潜在的N-糖基化位点和已知与细胞外糖胺聚糖相互作用的C-末端肝素结合基序（HBM）。通过使用宿主细胞结合试验，重组SWP 26蛋白（rSWP 26）可以抑制孢子粘附10%，从而降低宿主细胞感染。相反，突变体rSWP 26（r Delta SWP 26，不含HBM）在抑制孢子粘附方面无效。免疫电镜观察表明，该蛋白在孢子发育过程中主要表达于孢子内壁和质膜，而在成熟孢子的外壁中则有少量表达。目前的研究结果表明，SWP 26是一种微孢子虫细胞壁蛋白，参与内生孢子的形成，宿主细胞粘附和体外感染。SWP 26可作为家蚕微粒子病的诊断研究和药物设计的一个很有前景的靶标。(C)2008年澳大利亚寄生虫学会由Elsevier Ltd.出版。保留所有权利。
Microsporidia are obligate intracellular parasites related to fungi with resistant spores against various environmental stresses. The rigid spore walls of these organisms are composed of two major layers, which are the exospore and the endospore. Two spore wall proteins (the endosporal protein-SWP30 and the exosporal protein-SWP32) have been previously identified in Nosema bombycis. In this study, using the MALDI-TOF-MS technique, we have characterised a new 25.7-kDa spore wall protein (SWP26) recognised by monoclonal antibody 2G10. SWP26 is predicted to have a signal peptide, four potential N-glycosylation sites, and a C-terminal heparin-binding motif (HBM)which is known to interact with extracellular glycosaminoglycans. By using a host cell binding assay, recombinant SWP26 protein (rSWP26) can inhibit spore adherence by 10%, resulting in decreased host cell infection. In contrast, the mutant rSWP26 (r Delta SWP26, without HBM) was not effective in inhibiting spore adherence. Immuno-electron microscopy revealed that this protein was expressed largely in endospore and plasma membrane during endospore development, but sparsely distributed in the exospore of mature spores. The present results suggest that SWP26 is a microsporidia cell wall protein that is involved in endospore formation, host cell adherence and infection in vitro. Moreover, SWP26 could be used as a good prospective target for diagnostic research and drug design in controlling the silkworm, Bombyx mori, pebrine disease in sericulture. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.