Creating a spectrum of fibrocartilages through different cell sources and biochemical stimuli
Creating a spectrum of fibrocartilages through different cell sources and biochemical stimuli
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DOI：
10.1002/bit.21768
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发表时间：
2008-06-15
影响因子：
3.8
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Hoben, Gwendolyn A.;Athanasiou, Kyriacos A.
在这项研究中，采用两种不同的细胞来源和生物化学刺激的无支架方法来设计一系列代表膝关节半月板不同区域的纤维软骨。将由100%纤维软骨细胞（FC）或纤维软骨细胞和软骨细胞（CC）的50：50共培养物组成的构建体在10%胎牛血清培养基或无血清“软骨形成”培养基中培养，各+/-10 ng/mL TGF-β 1（+T）。将来自这两个细胞组和四种培养条件的构建体培养6周。通过改变细胞类型和生长因子的存在，每干重构建体的GAG跨越天然组织的GAG，在CC和FC组中分别为16-45%和1-7%。胶原密度最依赖于细胞类型，并显着低于组织值。胶原蛋白I/II比率可以通过细胞类型和血清存在来操纵，以跨越天然范围，从无血清CC组的3.5到用含血清培养基处理的FC组的超过1，000。在无血清培养基存在下使用CC细胞组将压缩刚度显著增加至128 +/- 34 kPa，与天然组织相似。类似地，无血清培养基或TGF-β 1处理将拉伸模量提高了一个数量级，高达3，000 kPa。使用两种细胞来源和操纵生化刺激，一系列的纤维软骨新组织工程。纤维软骨，如膝关节半月板的特点是异质性的基质和功能特性，这项工作表明了一种策略，重建这些异质组织。
In this study a scaffoldless approach was employed with two different cell sources and biochemical stimuli to engineer a spectrum of fibrocartilages representative of the different regions of the knee meniscus. Constructs composed of 100% fibrochondrocytes (FC) or a 50:50 co-culture of fibrochondrocytes and chondrocytes (CC) were cultured in 10% fetal bovine serum medium or serum-free "chondrogenic" medium, each +/-10 ng/mL TGF-beta 1 (+T). Constructs from these two cell groups and four culture conditions were cultured for 6 weeks. By varying the cell type and presence of the growth factor, GAG per dry weight of the constructs spanned that of native tissue, ranging 16-45% and 1-7% in the CC and FC groups, respectively. Collagen density was most dependent on cell type and was significantly lower than tissue values. The collagen I/II ratio could be manipulated by cell type and serum presence to span the native range, from 3.5 in the serum-free CC group to over 1,000 in the FC groups treated with serum-containing medium. Using the CC cell group in the presence of serum-free medium dramatically increased the compressive stiffness to 128 +/- 34 kPa, similar to native tissue. Similarly, serum-free medium or TGF-beta 1 treatment enhanced the tensile modulus by an order of magnitude, up to 3,000 kPa. Using two cell sources and manipulating biochemical stimuli, a range of fibrocartilaginous neotissues was engineered. Fibrocartilages such as the knee meniscus are characterized by heterogeneity in matrix and functional properties, and this work demonstrates a strategy for recreating these heterogeneous tissues.