A novel type 11 secretion system in Pseudomonas aeruginosa
A novel type 11 secretion system in Pseudomonas aeruginosa
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DOI：
10.1046/j.1365-2958.2002.02759.x
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发表时间：
2002-01-01
影响因子：
3.6
通讯作者：
Filloux, A
中科院分区：
文献类型：
--
作者：
Ball, G;Durand, É;Filloux, A
铜绿假单胞菌菌株PAO 1的基因组序列已被确定，以促进后基因组研究，旨在了解这种无处不在的机会致病菌的适应能力。铜绿假单胞菌产生毒素和水解酶，其通过使用Xcp机制或“分泌物”的II型分泌途径分泌。在这项研究中，我们描述了一个新的基因簇，称为hxc，与xcp同源。在磷酸盐限制培养基中生长的hxcR突变体的表征揭示了在野生型或xcp衍生物突变株的培养上清液中发现的40 kDa蛋白的存在。该蛋白对应于碱性磷酸酶L-AP，重命名为LapA，其以xcp-独立但hxc-依赖的方式分泌。最后，我们发现hxc基因簇的表达受磷酸盐调节。这是第一次报告存在两个功能的II型分泌途径在同一个有机体，这可能与铜绿假单胞菌的高适应潜力。
The genome sequence of Pseudomonas aeruginosa strain PAO1 has been determined to facilitate post-genomic studies aimed at understanding the capacity of adaptation of this ubiquitous opportunistic pathogen. P aeruginosa produces toxins and hydrolytic enzymes that are secreted via the type II secretory pathway using the Xcp machinery or 'secreton'. In this study, we characterized a novel gene cluster, called hxc for homologous to xcp. Characterization of an hxcR mutant, grown in phosphate-limiting medium, revealed the absence of a 40 kDa protein found in the culture supernatant of wild-type or xcp derivative mutant strains. The protein corresponded to the alkaline phosphatase L-AP, renamed LapA, which is secreted in an xcp-independent but hxc-dependent manner. Finally, we showed that expression of the hxc gene cluster is under phosphate regulation. This is the first report of the existence of two functional type II secretory pathways within the same organism, which could be related to the high adaptation potential of P. aeruginosa.