Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization
Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization
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DOI：
10.1016/s0145-2126(00)00125-9
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发表时间：
2001-04-01
影响因子：
2.7
通讯作者：
Decker, HJ
中科院分区：
文献类型：
--
作者：
Naumann, S;Reutzel, D;Decker, HJ
本研究结合了常规细胞遗传学、荧光原位杂交（FISH）、多重FISH和比较基因组杂交（CGH）。在人类白血病细胞系K562上应用这种多模式方法时，染色体组成被详细地细化，并与文献中的数据进行比较。亚三倍体核型的众数为67，和21个独特的标记染色体进行了鉴定。六个标记物的分类与已发表的数据相同，并且可以首次完全澄清来自文献的另外五个标记物的组成。另外五个标记的组成，在不同的研究中以不同的方式解释，毫无疑问地得到了阐明。最后，我们研究的五个新标记在以前的研究中似乎没有等价物，很可能是由于传统细胞遗传学的局限性。本研究中所示的互补技术的组合应用将非常有用，为染色体水平上的精细基因型分析提供基础。(C)2001爱思唯尔科技有限公司版权所有。
This study combines conventional cytogenetics, fluorescence in situ hybridization (FISH), multiplex-FISH and comparative genomic hybridization (CGH). In applying this multimodal approach on the human leukemia cell line K562, the chromosome composition was refined in detail and compared with data from the literature. A hypotriploid karyotype with a modal chromosome number of 67, and 21 unique marker chromosomes were identified. The classification of six markers was identical to published data and the composition of five further markers from the literature could be fully clarified for the first time. The composition of another five markers, which have been interpreted in divergent ways in different studies, were elucidated without doubt. Finally, five new markers of our study seem to have no equivalents in former studies, very likely due to limitations of conventional cytogenetics. The combinatory application of complementary techniques as shown in this study will be very useful to provide the basis of a refined genotype analysis on the chromosomal level. (C) 2001 Elsevier Science Ltd. All rights reserved.