PHOSPHORUS METABOLITE CHARACTERIZATION OF HUMAN PROSTATIC ADENOCARCINOMA IN A NUDE-MOUSE MODEL BY P-31 MAGNETIC-RESONANCE SPECTROSCOPY AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
PHOSPHORUS METABOLITE CHARACTERIZATION OF HUMAN PROSTATIC ADENOCARCINOMA IN A NUDE-MOUSE MODEL BY P-31 MAGNETIC-RESONANCE SPECTROSCOPY AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
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DOI：
10.1002/nbm.1940050405
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发表时间：
1992-07-01
影响因子：
2.9
通讯作者：
NARAYAN, P
中科院分区：
文献类型：
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作者：
KURHANEWICZ, J;DAHIYA, R;NARAYAN, P
进行了一系列实验，以使用P-31 MRS和HPLC鉴定和定量DU 145异种移植物（在裸鼠中生长的人前列腺腺癌细胞系）的磷代谢物。将DU 145异种移植物的P-31光谱特征与灌注的DU 145细胞和原位人前列腺癌进行比较。这些研究表明，DU 145异种移植物和灌注的DU 145细胞均表现出相对于原位人前列腺腺癌谱的磷酸肌酸水平降低。在DU 145异种移植物和原位人前列腺癌的P-31光谱中观察到磷酸化激素（PME）水平升高。DU 145异种移植物的PME共振的主要成分被鉴定为磷酸胆碱和磷酸乙醇胺。DU 145异种移植物和灌注DU 145细胞中始终观察到高水平的二磷酸二酯（DPDE），但在原位原发性人腺癌的P-31谱中不存在。与光谱结果一致，手术切除的人体组织的高压液相色谱分析含有不显著量的DPDE，而DU 145异种移植物具有高水平的DPDE，主要由尿苷-5 '-二磷酸-N-乙酰半乳糖胺（22.4 nmol/mg蛋白质）和尿苷-5'-二磷酸-N-乙酰葡糖胺（7.4 nmol/mg蛋白质）组成。
A series of experiments were conducted to identify and quantify the phosphorus metabolites of DU 145 xenografts (a human prostatic adenocarcinoma cell line grown in nude mice) using P-31 MRS and HPLC. The P-31 spectral characteristics of DU 145 xenografts were compared to perfused DU 145 cells and to in situ human prostatic adenocarcinomas. These studies demonstrated that both DU 145 xenografts and perfused DU 145 cells exhibited reduced levels of phosphocreatine relative to spectra of in situ human prostatic adenocarcinomas. Elevated levels of phosphomonesters (PMEs) were observed in P-31 spectra of both DU 145 xenografts and in situ human prostatic adenocarcinomas. The major components of the PME resonance of DU 145 xenografts were identified as phosphocholine and phosphoethanolamine. High levels of diphosphodiesters (DPDEs) were consistently observed for both DU 145 xenografts and perfused DU 145 cells, but were absent in P-31 spectra of in situ primary human adenocarcinomas. In agreement with spectroscopic results, high pressure liquid chromatographic analyses of human tissue removed at surgery contained insignificant amounts of DPDEs while DU 145 xenografts had high levels of DPDEs consisting mainly of uridine-5'-diphospho-N-acetylgalactosamine (22.4 nmol/mg protein) and uridine-5'-diphospho-N-acetylglucosamine (7.4 nmol/mg protein).