Free sphingoid bases in normal murine tissues.
Free sphingoid bases in normal murine tissues.
复制标题
正常小鼠组织中存在游离鞘氨醇碱。
DOI：
10.1111/j.1432-1033.1988.tb13952.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Ikuo Goto
中科院分区：
文献类型：
--
作者：
Takurou Kobayashi;Kunihiko Mitsuo;Ikuo Goto
通过邻苯二甲醛衍生化和使用高效液相色谱法，在小鼠组织中检测到被认为不是天然存在的游离鞘氨醇碱。浓度为10-30 pmol/mg组织。肺含有最多的鞘氨醇碱。在类鞘氨醇碱的分子种类中，最丰富的是C18-鞘氨醇碱，其次是C18-鞘氨醇碱、4-羟基鞘氨醇碱和C20-鞘氨醇碱。中枢神经组织含有相对较高量的C20-鞘氨醇，肾脏中有高浓度的4-羟基鞘氨醇。此外，在脊髓和坐骨神经中同时检测到半乳糖鞘氨醇。采用脂质萃取、阳离子交换、柱层析、碱皂化和制备薄层层析等方法从正常小鼠肺组织中分离纯化鞘氨醇类生物碱。在纯化的鞘氨醇碱，赤型-C18-鞘氨醇和赤型-C18-鞘氨醇的鉴定使用薄层色谱，高效液相色谱和快速原子轰击质谱。存在于正常组织中的游离鞘氨醇碱可能是合成所需的代谢中间体，也可能是鞘脂降解的产物，并具有调节细胞代谢的功能。
Free sphingoid bases, which have been considered not to occur naturally, were detected in murine tissues by derivatization with o-phthalaldehyde and the use of high-performance liquid chromatography. The concentrations were 10-30 pmol/mg tissue. The lung contained the largest amounts of sphingoid bases. In the molecular species of sphingoid bases, the most abundant was C18-sphingenine followed by C18-sphinganine, 4-hydroxysphinganine and C20-sphingenine, in that order. The central nervous tissues contained relatively high amounts of C20-sphingenine and there was a high concentration of 4-hydroxysphinganine in the kidney. In addition, galactosylsphingenine was detected simultaneously in the spinal cord and sciatic nerve. Sphingoid bases were purified from normal murine lungs using lipid-extraction, cation-exchange and silicic acid column chromatographies, alkaline saponification and preparative thin-layer chromatography. In the purified sphingoid bases, erythro-C18-sphingenine and erythro-C18-sphinganine were identified using thin-layer chromatography, high-performance liquid chromatography and fast-atom-bombardment mass spectrometry. Free sphingoid bases occurring in normal tissues may be metabolic intermediates required for the synthesis or be products of degradation of the sphingolipids and function to regulate cellular metabolism.
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乙腈-盐酸水解神经节苷脂，对其长链碱基进行高效液相色谱分析。
影响因子：
6.5
作者：
Kadowaki,H;Bremer,EG;Evans,JE;Jungalwala,FB;McCluer,RH
通讯作者：
McCluer,RH
大脑中半乳糖基鞘氨醇（精神鞘氨醇）的分析。
影响因子：
6.5
作者：
Igisu,H;Suzuki,K
通讯作者：
Suzuki,K
DOI：
10.1016/s0021-9258(18)67134-0
发表时间：
1986-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Merrill;A. Sereni;V. Stevens;Y. Hannun;R. Bell;J. M. Kinkade
通讯作者：
A. Merrill;A. Sereni;V. Stevens;Y. Hannun;R. Bell;J. M. Kinkade
影响因子：
56.9
作者：
HANNUN, YA;BELL, RM
通讯作者：
BELL, RM
通过反相高效液相色谱分析鞘氨醇碱。
影响因子：
6.5
作者：
Jungalwala,FB;Evans,JE;Bremer,E;McCluer,RH
通讯作者：
McCluer,RH