Graphene oxide-based homogenous biosensing platform for ultrasensitive DNA detection based on chemiluminescence resonance energy transfer and exonuclease III-assisted target recycling amplification
Graphene oxide-based homogenous biosensing platform for ultrasensitive DNA detection based on chemiluminescence resonance energy transfer and exonuclease III-assisted target recycling amplification
复制标题
基于氧化石墨烯的同质生物传感平台，用于基于化学发光共振能量转移和核酸外切酶III辅助靶标循环放大的超灵敏DNA检测
DOI：
10.1039/c3tb20270d
复制
发表时间：
2013-01-01
影响因子：
7
通讯作者：
Li, Baoxin
中科院分区：
文献类型：
--
作者：
Chen, Chun;Li, Baoxin
我们报道了一种用于超灵敏DNA检测的放大化学发光（CL）生物传感平台。它是基于核酸外切酶III辅助的靶循环扩增和氧化石墨烯（GO）的超级淬灭效率。在靶DNA存在下，靶-探针杂交体形成双链结构，核酸外切酶III催化从荧光素标记的探针DNA中逐步去除单核苷酸，导致靶DNA的再循环和鲁米诺-H2 O2-HRP-荧光素化学发光共振能量转移（CRET）系统的CL信号放大。该传感器对靶DNA的检测限可低至9 fM，灵敏度比基于GO的荧光共振能量转移（FRET）传感器高3个数量级。这种CL生物传感器具有简便、灵敏、快速和成本效益高的优点。
We report an amplified chemiluminescence (CL) biosensing platform for ultrasensitive DNA detection. It is based on exonuclease III-assisted target recycling amplification and the super quenching efficiency of graphene oxide (GO). In the presence of target DNA, the target-probe hybrid forms a double-stranded structure, and exonuclease III catalyzes the stepwise removal of mononucleotides from the fluorescein-labeled probe DNA, resulting in the recycling of the target DNA and CL signal amplification of the luminol-H2O2-HRP-fluorescein chemiluminescence resonance energy transfer (CRET) system. The detection limit of target DNA was estimated to be as low as 9 fM, and the sensitivity was about 3 orders of magnitude better than that of GO-based fluorescence resonance energy transfer (FRET) sensors for DNA with exonuclease III-assisted amplification. This CL biosensor offers the advantages of being facile, sensitive, rapid and cost-effective.