Extraction of uricase from Candida utilis by applying polyethylene glycol (PEG)/ NH4)2SO4 aqueous two-phase system
Extraction of uricase from Candida utilis by applying polyethylene glycol (PEG)/ NH4)2SO4 aqueous two-phase system
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DOI：
10.5897/ajb10.412
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发表时间：
2010-07
影响因子：
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通讯作者：
Yong Chen;Yan-fa Meng;Jin-Bao Tang;Tongtong Sun;Fengxiang Sun;Shujuan Liang
中科院分区：
文献类型：
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作者：
Yong Chen;Yan-fa Meng;Jin-Bao Tang;Tongtong Sun;Fengxiang Sun;Shujuan Liang
The purification of uricase from Candida utilis was performed by applying aqueous two-phase system (ATPS). Based on polyethylene glycols (PEG)/(NH4)2SO4 ATPSs, purification fold and recovery rate were demonstrated to influence the system parameters, such as PEG molecular mass, PEG concentration, ammonium sulfate [(NH4)2SO4] concentration, inorganic concentration, pH and additional amount of crude enzyme. The ATPS formed by 25% (w/w) PEG2000, 9% (w/w) (NH4)2SO4, 2% (w/w) NaCl, pH 7.5 and addition of 5% (w/w) crude enzyme showed the best separation capability. These conditions led to the 93% (w/w) recovery of uricase with 10-fold purification.
