IL-6 plays an essential role in neutrophilia under inflammation
IL-6 plays an essential role in neutrophilia under inflammation
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DOI：
10.1016/j.cyto.2011.01.007
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发表时间：
2011-04-01
期刊：
影响因子：
3.8
通讯作者：
Mihara, Masahiko
中科院分区：
文献类型：
--
作者：
Hashizume, Misato;Higuchi, Yoshinobu;Mihara, Masahiko
在本研究中，我们探讨了炎症条件下白细胞介素-6 （IL-6）在嗜中性粒细胞中的作用。关节炎猴子外周血中性粒细胞计数高，抗il -6受体抗体使中性粒细胞计数降至正常水平。正常猴子注射IL-6后3 h，血液中中性粒细胞计数明显增加。IL-6可降低循环中性粒细胞中cd162的表达。IL-6体外处理不影响人血液中性粒细胞中cd162的表达。在il -6治疗的猴子中，血浆中IL-8和粒细胞-巨噬细胞集落刺激因子（GM-CSF）水平明显升高。IL-8和GM-CSF体外处理降低了人中性粒细胞细胞表面CD162的表达，并且增加了细胞上清中可溶性CD162的表达。在人全外周血中加入IL-6可诱导IL-8的产生并降低中性粒细胞中cd162的表达。此外。IL-8和GM-CSF增强了中性粒细胞中崩解素和金属蛋白酶样结构域10 （ADAM10）的mRNA表达。中性粒细胞样HL-60细胞中siRNA敲低ADAM10可部分逆转GM-CSF和IL-8在HL-60细胞上降低的CD162的表达。综上所述，IL-6可诱导中性粒细胞增多，降低炎症中中性粒细胞CD162的表达。(C) 2011 Elsevier Ltd.版权所有。
In the present study, we explored the involvement of interleukin-6 (IL-6) in neutrophilia under inflammatory conditions. The neutrophil count in the peripheral blood was high in arthritic monkeys, and anti-IL-6 receptor antibody reduced neutrophil counts to normal levels. IL-6 injection into normal monkeys significantly increased neutrophil counts in the blood 3 h after injection. The expression of cluster of differentiation (CD) 162 on circulating neutrophils was reduced by IL-6 injection. IL-6 treatment in vitro did not affect CD 162 expression on neutrophils from human blood. In IL-6-treated monkeys, IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF) levels in plasma were clearly elevated. IL-8 and GM-CSF treatment in vitro reduced cell-surface CD162 expression on human neutrophils, and moreover, increased soluble CD162 expression in the cell supernatant. The addition of IL-6 into human whole peripheral blood induced IL-8 production and reduced CD 162 expression on neutrophils. Furthermore. IL-8 and GM-CSF augmented mRNA expression of a disintegrin and metalloprotease like domain 10 (ADAM10) in neutrophils. Knock-down of ADAM10 by siRNA in neutrophil-like HL-60 cells partially reversed the expression of CD162 reduced by GM-CSF and IL-8 on HL-60 cells. In conclusion, IL-6 induced neutrophilia and reduced CD162 expression on neutrophils in inflammation. (C) 2011 Elsevier Ltd. All rights reserved.