Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
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DOI：
10.2807/1560-7917.es.2020.25.9.2000152
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发表时间：
2020-03-05
期刊：
影响因子：
19
通讯作者：
Luetgehetmann, Marc
中科院分区：
文献类型：
--
作者：
Pfefferle, Susanne;Reucher, Svenja;Luetgehetmann, Marc
面对严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）的出现，全世界的实验室都需要进行大量的呼吸道检测。我们评估了在高通量平台cobas 6800上检测SARS-CoV-2的分子检测方法的性能，该平台使用“开放通道”来整合实验室开发的检测方法。我们在临床标本中观察到良好的分析性能。全自动化的工作流程能够以最少的动手时间进行高通量测试，同时提供快速可靠的结果。
Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the 'open channel' for integration of a laboratory-developed assay. We observed good analytical performance in clinical specimens. The fully automated workflow enables high-throughput testing with minimal hands-on time, while offering fast and reliable results.