Two DNA sequences specific for the canine Y chromosome
Two DNA sequences specific for the canine Y chromosome
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DOI：
10.1046/j.1365-2052.1998.00299.x
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发表时间：
1998-04-01
期刊：
影响因子：
2.4
通讯作者：
Lust, G
中科院分区：
文献类型：
--
作者：
Olivier, M;Lust, G
提供了专门在公狗DNA中发现的两个核苷酸序列的特征数据。在用任意序列的十核苷酸引物(OP-W17)对犬基因组DNA进行聚合酶链式反应(PCRS)时，只从雄性样本中扩增出650和990个核苷酸片段，而从雄性和雌性样本中都能扩增出400~2500个核苷酸片段。对这两个雄性特异片段进行克隆和测序，合成末端24mer的寡核苷酸引物对。用这些特定的引物对只从34只公狗的DNA样本中扩增出两个雄性特异序列，而从42只雌性狗的DNA样本中没有扩增出产物。先前位于Y染色体上的一段SRY基因可以在含有这两个新序列的DNA样本中扩增出来。当EcoRI将雄性DNA与650bp或990bp的序列杂交时，在Southern分析中各有一条带；雌性的DNA没有产生任何条带。将这两个新序列与SRY基因片段进行比较，没有发现同源性。我们得出的结论是，这两个新序列是犬Y染色体所特有的，不包含SRY基因的短特征片段。
Data are presented on the characterization of two nucleotide sequences found exclusively in the DNA of male dogs. In polymerase chain reactions (PCRs) of canine genomic DNA with a decanucleotide primer of arbitrary sequence (OP-W17), two nucleotide segments (650 and 990 bp) were amplified only from male samples, whereas a number of other fragments between 400 and 2500 bp in size were amplified from both male and female samples. The two male-specific segments were cloned and sequenced, and terminal 24mer oligonucleotide primer pairs were synthesized. PCR with these specific primer pairs resulted in amplification of the two male-specific sequences only from DNA samples of 34 male dogs; no product was amplified from 42 samples of females. A segment of the SRY gene previously localized on the Y chromosome could be amplified in DNA samples that had the two new sequences. EcoRI digested genomic male DNA when hybridized with the 650 bp or the 990 bp sequences, resulted in a single band for each on Southern analysis; DNA from females did not yield any bands. Comparisons between the two new sequences and the SRY gene segment revealed no homologies. We concluded that the two new sequences are specific for the canine Y chromosome and do not contain the short characterized segment of the SRY gene.