Influence of outer surface protein A antibody on Borrelia burgdorferi within feeding ticks
Influence of outer surface protein A antibody on Borrelia burgdorferi within feeding ticks
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DOI：
10.1128/iai.67.1.30-35.1999
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发表时间：
1999-01-01
影响因子：
3.1
通讯作者：
Fikrig, E
中科院分区：
文献类型：
--
作者：
de Silva, AM;Zeidner, NS;Fikrig, E
伯氏疏螺旋体是莱姆病的螺旋体病原体，由硬蜱传播，当受感染的若虫以宿主为食时，体内的细菌数量增加，然后它们入侵硬蜱的唾液腺并感染宿主。针对伯氏杆菌外表面蛋白A(OspA)的抗体可杀灭喂食硬壳类体内的螺旋体，并阻断向宿主的传播。在这里介绍的研究中，进行了被动抗体转移实验，以确定阻断向啮齿动物宿主传播所需的OspA抗体效价。OspA抗体水平是通过竞争的酶联免疫吸附试验来测定的，该分析测量了抗体与单抗C3.78所定义的保护性表位的结合，C3.78 OspA抗体效价(~gt；213mU/ml)显著高于阻断向宿主传播所需的效价(~gt；6mU/ml)，尽管在Laffer抗体水平上没有根除硬蜱中的螺旋体，但抗体减少了喂食性扁虱内的螺旋体的数量，并干扰了螺旋体诱导脊髓炎和侵入载体唾液腺的能力。OspA抗体可能会直接干扰伯氏杆菌侵入媒介唾液腺的能力；或者，OspA抗体可能会将喂食扁虱的螺旋体密度降低到启动与传播相关的事件所需的临界阈值以下。
Borrelia burgdorferi, the spirochetal agent of Lyme disease, is transmitted by Ixodes ticks, When an infected nymphal tick feeds on a host, the bacteria increase in number within the tick, after which they invade the ticks salivary glands and infect the host. Antibodies directed against outer surface protein A (OspA) of B. burgdorferi kill spirochetes within feeding ticks and block transmission to the host. In the studies presented here, passive antibody transfer experiments were carried out to determine the OspA antibody titer required to block transmission to the rodent host. OspA antibody levels were determined by using a competitive enzyme-linked immunosorbent assay that measured antibody binding to a protective epitope defined by monoclonal antibody C3.78, The C3.78 OspA antibody titer (>213 mu g/ml) required to eradicate spirochetes from feeding ticks was considerably higher than the titer (>6 mu g/ml) required to block transmission to the host, Although spirochetes were not eradicated from ticks at laffer antibody levels, the antibodies reduced the number of spirochetes within the feeding ticks and interfered with the ability of spirochetes to induce ospC and invade the salivary glands of the vector. OspA antibodies may directly interfere with the ability of B. burgdorferi to invade the salivary glands of the vector; alternately, OspA antibodies may lower the density of spirochetes within feeding ticks below a critical threshold required for initiating events linked to transmission.